Generating Skin-Derived Precursor-Like Cells From Human-Induced Pluripotent Stem Cell-Derived Skin Organoids

November 2024 in “ Experimental Dermatology ”

I. Ahmed, Jane Sun, Muwen Kong, Kiarash Khosrotehrani, Abbas Shafiee

skin-derived precursor-like cells human-induced pluripotent stem cells skin organoids SOX2 fibronectin S100β hair regenerative applications progenitor cells SKP-like cells iPSCs SKOs

Studysummary In this pilot study, researchers isolated skin-derived precursor-like cells from human-induced pluripotent stem cell-derived skin organoids, revealing their potential for future hair regenerative applications by exhibiting self-renewal and key marker expression.

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This study explores the potential of generating skin-derived precursor-like (SKP-like) cells from human-induced pluripotent stem cell-derived skin organoids (SKOs) to overcome the limitations of adult human SKPs, which lose potency after in vitro expansion. The researchers developed a protocol to isolate SKP-like cells from SKOs, which maintained characteristic spheroid morphology and self-renewal capabilities. Immunofluorescence analysis confirmed the expression of key markers such as SOX2, fibronectin, and S100β in these cells. The findings suggest that SKO-derived SKP-like cells could be promising for hair regenerative applications and highlight the value of SKOs as a source for isolating progenitor cells to advance hair regeneration and restore skin function.

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