Bioengineering of Hair Follicle-Like Structure for Validation of Hair Growth Promoting Compounds

In this study, hair follicle germs containing vascular endothelial cells showed improved hair regeneration and morphogenesis-related gene expression in mice, suggesting a promising strategy for hair regenerative medicine.

This study introduced a simple and reliable in vitro assay capable of large-scale simultaneous screening of hair growth-promoting compounds using a 3D co-culture system of human dermal papilla and outer root sheath cells.

This study explored the use of "microfollicles," a human hair follicle organoid model, as an in vitro tool for testing hair-growth substances, finding up to 75% overlap in gene expression changes with human clinical biopsy responses to minoxidil, indicating its potential for pre-clinical substance testing.

Scientists created human skin with hair from stem cells, which could help treat hair loss and skin conditions.

This study demonstrates that incorporating human neopapilla into reconstructed human skin can initiate hair follicle morphogenesis by stimulating epidermal invagination and maintaining follicle-inductive properties in vitro.

This study found that 3D cultured hair follicle-like constructs, which include keratinocytes and dermal papilla cells, showed improved gene expression and cell interactions relevant to hair follicle development.

This review discusses methods for evaluating hair growth-promoting effects of candidate substances for androgenetic alopecia, focusing on cell assays, organ cultures, and animal models, and reports no new results.

This study demonstrated that enhancing human skin constructs with hair follicles through engineered cell organization and vascularization improved hair growth in immunodeficient mice, suggesting potential advances for treating alopecia and chronic wounds.

This study found that alginate spheres maintain dermal papilla cells in a dormant state with increased trichogenecity, suggesting potential benefits for cell therapy in androgenic alopecia.

This study suggests that co-culturing human outer root sheath cells with human dermal papilla cells can restore their ability to induce hair growth by altering gene expression through paracrine signaling.

This study observed that applying JAK-STAT pathway inhibitors to mouse and human skin led to rapid hair growth by inducing the anagen phase of hair follicles.

This review provides a comprehensive guide to serum-free human hair follicle organ culture methods, highlighting research opportunities, standardization efforts, and potential applications without reporting new empirical findings.

This review summarizes causes and treatments of androgenetic alopecia with a focus on herbs as potential alternatives, but reports no new results and suggests more research is needed to validate these options.

In this study, researchers found that the number of dermal papilla cells in hair follicles determines hair size and shape in mice, suggesting potential avenues for addressing hair loss.

This study successfully established an immortalized human dermal papilla cell line that maintains characteristics of original cells without malignant transformation, potentially benefiting hair research.

This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

This study successfully recreated human hair follicles in vitro, which offers a potential advancement in hair regeneration and understanding the effects of drugs on hair follicles.

This review summarizes fundamental concepts and recent advancements in hair follicle biology, including insights from mouse models into broader molecular and cellular processes relevant to regeneration and development.

In this study, co-grafting human keratinocytes with murine dermal papilla-enriched cells produced hair follicle-like structures, but without regular hair formation, suggesting anomalous folliculogenesis.

This article reviews the molecular interactions involved in hair follicle cycling and highlights the role of dermal papilla cells, but it presents no new research findings.

This article reviews the mechanisms by which minoxidil stimulates hair growth and reports no new clinical findings.

This study found that the volume of a hair follicle's dermal papilla is strongly correlated with its size, primarily due to the number of cells it contains and the volume of extracellular matrix.