A Two-Stepped Culture Method for Efficient Production of Trichogenic Keratinocytes
October 2015
in “Tissue Engineering Part C-methods”
TLDR A two-step method was created in 2015 to make more cells that help with hair growth, but they need to be combined with other cells for 4 days to actually form new hair.
In 2015, researchers developed a two-step culture method to efficiently produce trichogenic keratinocytes, cells vital for hair growth. Initially, keratinocytes were cultivated in a low calcium concentration, then transferred to a high calcium concentration, resulting in a higher yield of these cells. The study found that keratinocytes could be expanded for over 100 passages without losing their proliferative potential, but they were unable to form new hair follicles (HFs) unless cocultured with HF dermal papilla (DP) cells for 4 days. This coculture enhanced Wnt/β-catenin signaling, crucial for HF development, and upregulated the expression of HF-specific genes. This method could potentially offer a more effective approach for treating hair loss conditions such as alopecia.
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