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This study found that an enzymatic digestion method for isolating hair follicle stem cells from scalp specimens yielded 5.3 times more epithelial cells than explant cultures, while supporting comparable cell functionality, offering a promising technique for tissue engineering and regenerative medicine.

This study adapted a method for culturing melanocytes from human hair follicles to horses, successfully creating partially melanotic equine melanocytes, potentially advancing hair follicle biology research across species.

This study established a method to create stem cell-derived dermal papilla cells and found that their conditioned medium significantly boosts hair growth and offers anti-inflammatory benefits in mice, surpassing minoxidil and addressing androgen-related hair loss.

This study describes a method for labeling and tracking hair follicle stem cells in mouse skin using a Cre/lox recombination system with tamoxifen, providing insights into stem cell behavior and contribution to tissue regeneration.

This study found that a prevascularized collagen fiber space improved hair generation compared to intracutaneous transplantation by providing a better microenvironment for transplanted cells.

In this study, hair follicle germs containing vascular endothelial cells showed improved hair regeneration and morphogenesis-related gene expression in mice, suggesting a promising strategy for hair regenerative medicine.

This study reviewed the efficacy of mechanical fractionation techniques to produce tissue stromal vascular fraction (tSVF) and found that tSVF injections are particularly effective for conditions like osteoarthritis and wound healing, with centrifugation before fractionation improving isolation efficiency regardless of preparation method.

This study developed an efficient method for isolating and enriching multipotent ovine hair follicle stem cells, which may aid in research on the ovine hair cycle and future wool production.

This study demonstrated that customized microneedle arrays can precisely induce targeted hair regeneration and improve hair quality in mice, potentially offering a personalized treatment approach for hair loss.

This article describes a protocol for using bioengineered techniques to regenerate functional hair follicles and their stem cell niches by manipulating epithelial and mesenchymal cells.

This study reviews current methods for making and using platelet-rich plasma and fibrin, emphasizing the variability in clinical outcomes due to differences in preparation protocols, and calls for standardized guidelines and large-scale studies for better effectiveness in regenerative medicine.

In this study, researchers used a chemical cocktail and hydrogel microspheres to successfully guide fibroblasts into dermal papilla cells, promoting wound healing and in situ hair follicle regeneration while reducing scar formation, in both in vitro and in vivo experiments.

This study introduces "Homeodynamic Rejuvenation" as a method to restore the skin's resilience by enhancing its ability to detect and recover from stress, focusing on five core biological processes critical for maintaining skin function and health amidst environmental and metabolic challenges.

This study developed a simple method to prepare highly concentrated, resting platelet-rich plasma suitable for cell therapy, effectively preventing clotting while maintaining platelet reactivity and minimizing hemorrhage risk.

This review found that adipose-derived stem cells and stromal vascular fraction have robust clinical benefits in aesthetic surgery, particularly in facial rejuvenation, scar treatment, and volumetric restoration, with favorable safety profiles.

This study found that crosslinking buffalo gelatin with different methods can modify its properties and potential as a skin substitute, though double-crosslinking may increase immunogenicity slightly.

This study proposes a two-step method that may enhance the generation of stem-like epidermal cells with superior proliferation and differentiation potential for skin regeneration.

This review highlights recent advances in CRISPR-based lineage tracing methods that can improve our understanding of skin stem cell behavior, regeneration, and disease, with potential applications in organoids and model organisms.

This study introduced a noninvasive spectroscopy method to assess hair follicle stages in mice by measuring skin diffuse reflectance and melanin dynamics, offering a potential tool for hair regeneration research.

In this study, researchers found that a cold atmospheric plasma-activated hydrogel containing interleukin-2 significantly enhanced hair regeneration in a depilated mouse model, promoting larger hair follicles and faster hair growth phase transition, suggesting it as a potential treatment for hair loss.

In this study, researchers developed methods to isolate and culture rat hair follicle stem cells, confirming their potential for skin regeneration by analyzing growth characteristics and bioactive secretions, contributing to regenerative medicine advancements.

This study presents a modified method for synthesizing ~1 nm zinc oxide nanoparticles within the pores of SBA-11, maintaining the mesoporous structure without requiring alcohol reflux or basic solution reaction steps.

This study found that alkylation of kerateine cysteine residues created a cell-compatible method for controlling hydrogel erosion rates and therapeutic agent release in keratin-based systems.

This protocol describes using two-photon laser-scanning microscopy to study hair regeneration in live mice, offering detailed imaging capabilities for observing hair follicle stem cell behavior and manipulating cellular populations.

In this study, researchers used a new method with platelet-rich plasma-loaded microcarriers to enhance dermal papilla cell activity and hair follicle regeneration, achieving significant hair and vessel growth in a mouse model compared to control groups.

This review found preliminary evidence suggesting that photothermal conditioning enhances the bioactivity of platelet-rich plasma, potentially improving hair regeneration outcomes, but highlights the need for standardized protocols and further studies to confirm its efficacy and safety.

This study reported the successful isolation of epithelial stem cells from mouse embryonic skin using a simple culture method, with the cells showing the potential to differentiate into multiple cell types.

This study found that the patch assay effectively reconstituted mature hair follicles using culture-expanded human fetal cells and may serve as a method to assess cell trichogenicity.

In this study, researchers developed a microfluidics-based method to generate hair follicle germs on a large scale for potential hair regeneration therapies, demonstrating efficient hair follicle regeneration following transplantation into mice.

In this study, the researchers reported that using a biodegradable ECM scaffold for large-area wound regeneration accelerated wound coverage and improved hair follicle neogenesis by activating the adaptive immune system.