Spectrofluorimetric Study of Finasteride and Bovine Serum Albumin Interaction and Its Application for Quantitative Determination of Finasteride in Tablet Dosage Form

    January 2015 in “ Analytical Methods ”
    Ali S. Abdelhameed, Amer M. Alanazi, Adnan A. Kadi
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    TLDR Method accurately measures finasteride in tablets using finasteride-BSA interaction.
    This paper describes a spectrofluorimetric method for measuring finasteride in tablet dosage form using the interaction between finasteride and bovine serum albumin (BSA). The method is accurate, precise, and sensitive, with a limit of detection of 0.16 μg mL-1 and a limit of quantification of 0.49 µg mL-1. The study also determined the binding mode and binding sites of finasteride and BSA, as well as the thermodynamic parameters and binding forces of the interaction. The method provides a quantitative determination of finasteride in tablet dosage form.
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