Integrated-Omics Profiling Unveils the Disparities of Host Defense to ECM Scaffolds During Wound Healing in Aged Individuals

The study investigated the effectiveness of extracellular matrix (ECM) scaffold membranes in wound healing, comparing younger and aged mice. Using single-cell RNA sequencing and spatial transcriptomics, researchers found that aged mice had poorer outcomes in re-epithelialization and collagen deposition. The presence of senescent SPP1+ macrophages in aged mice was identified as a key factor impeding the activation of type L immune response, thus inhibiting the repair ability of epidermal cells and fibroblasts. These insights highlight the need for precision-based biomaterials tailored for aged individuals to improve therapeutic strategies.