Cytokine Loaded Layer-By-Layer Ultrathin Matrices to Deliver Single Dermal Papilla Cells for Spot-By-Spot Hair Follicle Regeneration

December 2015 in “ Journal of Materials Chemistry B ”

Bojie Lin, Jin Wang, Yong Miao, Yuqing Liu, Wei Jiang, Zhiqi Hu, Mohammad Ali Darabi, Zhiqi Hu, Malcolm Xing

layer-by-layer encapsulation gelatin alginate dermal papilla cells hair follicle growth hair follicle regeneration Fibroblast Growth Factor-2 FGF-2 hair reconstitution assays hair-inductivity LBL encapsulation DPCs

Studysummary This study found that using layer-by-layer encapsulation techniques on dermal papilla cells can support their function and may help treat hair loss when used in conjunction with freshly isolated epidermal cells. Our plain-language summary of this paper — not a Tressless recommendation.

In 2016, researchers developed a layer-by-layer (LBL) encapsulation technique using gelatin and alginate to deliver single dermal papilla cells (DPCs), crucial for hair follicle growth and regeneration. The encapsulation, which lasted about 10 days, did not affect the viability, proliferation, morphology, and intrinsic properties of DPCs, and may have induced a more dermal papilla-like phenotype in the cells. The study also incorporated Fibroblast Growth Factor-2 (FGF-2) into the LBL encapsulation, which was released in a sustained, time-dependent manner over 7 days. In vivo hair reconstitution assays showed enhanced hair-inductivity when LBL-DPCs were combined with FGF-2, suggesting this method could be a potential therapeutic strategy for treating hair loss. The document did not provide the number of people involved in the study.

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