Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders
March 2013
in “
InTech eBooks
”
Confocal Laser Scanning Microscopy CLSM fluorescence probes skin drug delivery systems topical therapies skin diseases epidermis cellular uptake penetration depth non-invasive optical sectioning three-dimensional reconstructions high-resolution imaging confocal microscopy fluorescent markers skin treatments skin conditions skin layers cell absorption skin penetration non-invasive imaging 3D imaging high-res imaging
TLDR Confocal Laser Scanning Microscopy (CLSM) is a useful tool for studying how drugs interact with skin and diagnosing skin disorders, despite some limitations.
The document from 10 years ago discussed the use of Confocal Laser Scanning Microscopy (CLSM) for studying skin drug delivery systems and diagnosing skin disorders. CLSM, using fluorescence probes, allowed the evaluation of drug interactions with the biological system, their cellular uptake, penetration depth and routes into the skin, and the effect of topical therapies for skin diseases. It overcame traditional microscopy limitations by providing high-resolution imaging, non-invasive optical sectioning, and three-dimensional reconstructions. The document also highlighted the use of CLSM in diagnosing skin disorders and diseases, providing imaging of nuclear, cellular, and tissue architecture of the epidermis and underlying structures without a biopsy. Despite some limitations, such as potential damage to living cells due to high intensity laser irradiation, CLSM was considered a valuable tool in dermatology.
