Cell-Based Model Systems for Validation of Efficacy Claims for Cosmetic Ingredients

October 2022 in “ Cosmetics ”

Prachi Singh, Shruthi S. Bhat, Nikhil Kumar Singh, Babu U. Venkanna, Mohamed Rafiq, Raghavendra P. Rao

hair growth 5-alpha reductase human follicle dermal papilla cells outer root sheath cells skin aging anti-oxidant anti-inflammatory gene expression skin hydration skin rejuvenation barrier function psoriasis wound healing hyperpigmentation dermal papilla cells

TLDR Cell-based models help test if cosmetic ingredients really work for hair growth and skin health.

The document discusses the use of cell-based model systems to validate the efficacy of cosmetic ingredients, particularly those promoting hair growth and skin health. Hair growth is influenced by the proliferation of certain cells and the inhibition of the enzyme 5-alpha reductase. The document also discusses the use of human follicle dermal papilla cells and outer root sheath cells to assess the effectiveness of cosmetic ingredients on hair growth. Skin aging is evaluated through cell-based assays based on anti-oxidant and anti-inflammatory activities, gene expression levels, and the effect on certain enzymes. The document also discusses the use of cell-based models to evaluate skin hydration, rejuvenation, barrier function, psoriasis, wound healing, and hyperpigmentation. The document also highlights the benefits and limitations of cell-based assays in screening cosmetic products.

View this study on mdpi.com →

Discuss this study in the Community →

Research cited in this study

15 / 15 results

research Effect of Coffee Berry Extract on Anti-Aging for Skin and Hair: In Vitro Approach

8 citations, June 2022 in “Cosmetics”

Coffee berry extract may help slow skin aging and prevent hair loss.

research Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells

13 citations, September 2021 in “Current Issues in Molecular Biology”

Dexpanthenol helps human hair follicle cells grow by preventing aging and death, and by supporting growth signals.

research Human Hair Outer Root Sheath Cells and Platelet-Lysis Exosomes Promote Hair Inductivity of Dermal Papilla Cells

9 citations, June 2020 in “Tissue Engineering and Regenerative Medicine”

HHORSC exosomes and PL improve hair growth treatment outcomes.

research Applications of Tea (Camellia Sinensis) and Its Active Constituents in Cosmetics

67 citations, November 2019 in “Molecules”

Tea, especially green tea, shows promise in cosmetics for skin and hair benefits but more research is needed for effective use.

research A New Label-Free Screen for Steroid 5α-Reductase Inhibitors Using LC-MS

15 citations, October 2016 in “Steroids”

Researchers developed a new method to find substances in herbs that can block a specific enzyme linked to hair loss.

research Androgens Induce Sebaceous Differentiation in Sebocyte Cells Expressing a Stable Functional Androgen Receptor

31 citations, April 2015 in “Journal of steroid biochemistry and molecular biology/The Journal of steroid biochemistry and molecular biology”

Androgens cause oil-producing skin cells with androgen receptors to mature and produce more oil.

research Hair-Growth-Promoting Effect of Conditioned Medium of High Integrin α6 and Low CD71 Positive Keratinocyte Cells

17 citations, February 2015 in “International Journal of Molecular Sciences”

A special mix from certain skin cells can help hair grow by making hair root cells grow faster and activating growth signals.

research Fibroblast Growth Factors Stimulate Hair Growth Through Beta-Catenin and Shh Expression in C57BL/6 Mice

64 citations, January 2015 in “BioMed Research International”

Certain growth factors can promote hair growth in mice by activating hair growth-related proteins.

Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus Acutissima Cortex

research Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus Acutissima Cortex

37 citations, January 2015 in “Evidence-based Complementary and Alternative Medicine”

An extract from Quercus acutissima bark was found to reduce sebum production and block an enzyme linked to acne.

research The Clock Gene Brain And Muscle Arnt-Like Protein-1 (BMAL1) Is Involved In Hair Growth

9 citations, August 2013 in “Archives of Dermatological Research”

A gene called BMAL1 plays a role in controlling hair growth.

research Eclipta Alba Extract With Potential For Hair Growth Promoting Activity

121 citations, May 2009 in “Journal of Ethnopharmacology”

Eclipta alba extract may help hair grow similarly to Minoxidil.

The Silver Locus Product (Silv/gp100/Pmel17) as a New Tool for the Analysis of Melanosome Transfer in Human Melanocyte–Keratinocyte Co-Culture

research The Silver Locus Product (Silv/gp100/Pmel17) as a New Tool for the Analysis of Melanosome Transfer in Human Melanocyte–Keratinocyte Co-Culture

68 citations, March 2008 in “Experimental dermatology”

The new assay can track and measure melanosome transfer between skin cells, confirming filopodia's role in this process.

research Establishment of Type II 5α-Reductase Over-Expressing Cell Line as an Inhibitor Screening Model

12 citations, June 2007 in “Journal of steroid biochemistry and molecular biology/The Journal of steroid biochemistry and molecular biology”

A reliable model for screening type II 5α-reductase inhibitors was created and validated.

research Inhibition of 5α-Reductase Activity in SZ95 Sebocytes and HaCaT Keratinocytes In Vitro

24 citations, February 2007 in “Hormone and metabolic research”

The substance MK386 effectively blocked testosterone conversion and reduced cell growth in certain skin cells, but inhibiting 5α-reductase alone may not greatly improve acne.

research In Vivo and In Vitro Evaluation of Hair Growth Potential of Hibiscus Rosa-Sinensis Linn.

128 citations, September 2003 in “Journal of Ethnopharmacology”

Hibiscus rosa-sinensis leaf extract helps hair grow longer and faster.

Related Community Posts Join

6 / 1000+ results

community New and Interesting HairLoss Studies/Papers/Reviews

in Treatment 42 upvotes 4 years ago

Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

community Surprisingly thorough study on He-Shou-Wu/Fo Ti

in Research/Science 14 upvotes 1 year ago

He Shou Wu (Fo Ti) extract was found to prolong the hair growth phase, inhibit 5-alpha-reductase (like finasteride), reduce androgen receptors, and increase growth factors, potentially outperforming minoxidil in recovering hair follicle size after DHT exposure. Two compounds, emodin and TSG, are identified as responsible for these effects and warrant further investigation.

community Fats on your Head to Stop Hair Loss?

in Research/Science 140 upvotes 10 months ago

The conversation discusses the potential of long-chain unsaturated fatty acids, like oleic and linoleic acid, as an additional treatment for hair loss, which may inhibit the enzyme responsible for converting testosterone to DHT and promote hair growth. Users humorously suggest using oils topically and discuss other hair loss treatments, but the main focus is on the science behind fatty acids and their role in hair health.

community The Real Cause Of Androgenetic Alopecia

in Research/Science 668 upvotes 2 months ago

Androgenetic alopecia is caused by DHT affecting hair growth. Finasteride and minoxidil are used to manage hair loss by blocking DHT and promoting hair growth.

community COMPLETE OVERVIEW of the Treatment of androgenetic alopecia in men

26 upvotes 10 years ago

Male androgenetic alopecia is commonly treated with topical minoxidil and oral finasteride, both requiring continuous use. Other options include hair restoration surgery, dutasteride, light therapy, and camouflaging agents.

community Diffuse hair loss and scalp biopsies

in Research 692 upvotes 4 months ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

Similar Research