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Glossary Multiphoton Microscopy

uses multiple photons for deep, detailed tissue imaging

Multiphoton Microscopy (MPM) is an advanced imaging technique that allows scientists to view living tissues at a cellular level with high resolution. Unlike traditional fluorescence microscopy, MPM uses multiple photons of lower energy to excite fluorescent dyes, reducing photodamage and allowing deeper tissue penetration. This makes it particularly useful for studying complex biological structures, such as hair follicles and skin layers, in their natural state.

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research Application of multiphoton microscopy in dermatological studies: A mini-review

58 citations , November 2013 in “Journal of Innovative Optical Health Sciences”

This review discusses the development and application of multiphoton microscopy in dermatology, highlighting its advantages and potential future research directions without reporting new clinical results.

research Feature characterization of scarring and non-scarring types of alopecia by multiphoton microscopy

24 citations , September 2018 in “Lasers in Surgery and Medicine”

This study shows that multiphoton microscopy can non-invasively distinguish between scarring and non-scarring alopecia by identifying certain morphological features, but further research is needed to confirm its clinical utility.

research Multiphoton microscopic imaging of in vivo hair mouse skin based on two‐photon excited fluorescence and second harmonic generation

8 citations , September 2011 in “Scanning”

This study found that multiphoton microscopy effectively visualizes the microstructure of in vivo mouse skin, offering a clear view of various skin layers and components like corneocytes and collagen fibers.

research Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope

5 citations , January 2019 in “Methods in molecular biology”

In this study, researchers showed that multiphoton microscopy with fluorescent protein tagging enables single-cell imaging and tracking in live transgenic mice, offering new insights into cell dynamics in stem cell research compared to traditional histological methods.

research Multiphoton microscopic imaging of rabbit dorsal skin

1 citations , December 2014 in “Scanning”

This study used multiphoton microscopy to successfully visualize rabbit skin microstructure, highlighting its noninvasive potential for future skin research related to diseases and wound healing.

research 068 Association of systemic involvement with skin morphology assessed by multiphoton microscopy in pseudoxanthoma elasticum

October 2021 in “Journal of Investigative Dermatology”

This study found no significant difference in SARS-CoV2 IgG seroconversion rates between patients with immune-mediated inflammatory diseases treated with targeted immune-modulating therapies and those on conventional systemic treatments.

research In vivo multiphoton microscopy imaging of vitiligo (Conference Presentation)

March 2019

This pilot study employed in-vivo multiphoton microscopy to visualize pigment-producing melanocytes in vitiligo patients, aiming to enhance understanding of treatment impacts and potentially improve transplantation therapies.

research Feature characterization of scarring and non-scarring types of alopecia by multiphoton microscopy (Conference Presentation)

March 2018

This study shows that multiphoton microscopy can non-invasively distinguish scarring from non-scarring alopecia by identifying specific morphological features in hair follicle structures.

research Application of multiphoton microscopy in dermatological studies: A mini-review

January 2014

This review discusses the advancements and applications of multiphoton microscopy in dermatology, including its role in imaging skin disease and drug transport, and reports no new clinical results.

research Technical Advancement and Biomedical Application of Multiphoton Excitation Microscopy

January 2019 in “The Review of Laser Engineering”

This study observed that multiphoton excitation microscopy revealed changes in vascular structure and hair papillae in mice subcutaneous tissue following cyclophosphamide treatment for chemotherapy-induced alopecia.

research Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy

3 citations , July 2024 in “Annals of Biomedical Engineering”

This study found that multiphoton microscopy imaging allows for detailed observation and quantitative analysis of collagen alterations in the progression of endometrial cancer, potentially offering a faster, more accurate method for early diagnosis compared to current protocols.

research Can mesoporous nanoparticles promote bioavailability of topical pharmaceutics?

15 citations , April 2021 in “International Journal of Pharmaceutics”

This study found that smaller mesoporous silica particles (400-600 nm) accumulated in skin furrows, suggesting their potential as carriers for enhancing the delivery of topical medications like metronidazole.

research Multi-Modal Imaging to Assess the Follicular Delivery of Zinc Pyrithione

6 citations , May 2022 in “Pharmaceutics”

This study demonstrates that MPM-FLIM multimodal imaging can effectively assess the delivery and dissolution of zinc pyrithione particles in human skin follicles using different application techniques.

research Intradermal Delivery of Calcium Hydroxylapatite With Fractionated Ablation

1 citations , August 2024 in “Lasers in Surgery and Medicine”

This study observed that calcium hydroxylapatite can be successfully incorporated and retained in the dermis for at least six weeks after fractional laser treatment, with new collagen forming around it.

research Non-Invasive Techniques for Evaluating Alopecia Areata

June 2023 in “Mædica”

This review discusses various non-invasive methods for diagnosing alopecia areata, highlighting that trichoscopy signs like short vellus hairs and yellow dots are sensitive indicators of the condition, while advanced imaging techniques provide valuable information on hair follicle status and scalp inflammation.

research Label-free multiphoton imaging to assess neoadjuvant therapy responses in breast carcinoma

14 citations , January 2020 in “International Journal of Biological Sciences”

This study explored the use of multiphoton microscopy to monitor changes in tumors and surrounding tissues in breast cancer patients undergoing neoadjuvant chemotherapy, finding it may effectively detect treatment response at cellular levels without using exogenous contrast agents.

research Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

97 citations , December 2021 in “Cells”

This review outlines key considerations for designing fluorescence microscopy experiments in cell biology, discussing the importance of hardware availability, suitability of biological models, and imaging agents to achieve high-resolution and informative images.

research Streamlined intravital imaging approach for long-term monitoring of epithelial cell dynamics on an inverted confocal microscope

1 citations , May 2023 in “bioRxiv (Cold Spring Harbor Laboratory)”

In this study, researchers developed a custom-designed 3D-printed microscope stage insert for inverted confocal microscopes, enhancing the accessibility of long-term intravital imaging of live cell dynamics in live transgenic mice, particularly in the skin.

research Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

1 citations , June 2023 in “Journal of Visualized Experiments”

This study introduces a customizable 3D-printed microscope stage insert for inverted confocal microscopes, improving accessibility and usability of intravital microscopy for observing cell dynamics in live mice.

research Compartmentation of Mitochondrial and Oxidative Metabolism in Growing Hair Follicles: A Ring of Fire

40 citations , March 2017 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study visualized mitochondrial metabolism in cultured bovine hair follicles and plucked human hairs, finding that ROS and mitochondrial polarization correlate in specific hair follicle regions, particularly near keratin deposition sites.

research Exploring Mechanical Niches Identified by Hair Follicle Compression and How These Transduce Proliferation and Differentiation Cues in Epidermally Derived cells.

January 2019 in “Durham e-Theses (Durham University)”

This study found that JNK plays a dominant role in hair follicle matrix density-dependent proliferation and differentiation, suggesting that collagen bundling affects keratinocyte dynamics.

research Two‐photon microscopy for intracutaneous imaging of stem cell activity in mice

16 citations , September 2016 in “Experimental Dermatology”

This review provides guidance on using two-photon microscopy for real-time and long-term visualization of stem cell activity in live mice skin, focusing on its application in researching skin and stem cell biology.

research Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing

13 citations , September 2021 in “Communications Biology”

This study introduces a five-modal microscopy technique that allows label-free, in vivo observation and quantitative analysis of various wound healing processes.

research Live imaging of skin immunity using two-photon microscopy: a short review

January 2026 in “Inflammation and Regeneration”

This review highlights that two-photon excitation microscopy is a valuable tool for visualizing live skin structures and immune responses at high resolution, though its clinical use is hindered by safety and cost issues.

research Three-Dimensional Analysis of Cell Division Orientation in Epidermal Basal Layer Using Intravital Two-Photon Microscopy

31 citations , September 2016 in “PLoS ONE”

The researchers reported that in hairless mice, epidermal cell division orientations and epidermal thickness varied by body site, with dorsal and ear epidermis primarily dividing parallel to the basement membrane, unlike hind paw and tail epidermis.

research An Investigation of Hair and its Keratin Associated Proteins using Advanced Light Microscopy

January 2019 in “Durham e-Theses (Durham University)”

This study utilized advanced imaging techniques to quantify hair damage and dye uptake dynamics, revealing that compound penetration in hair is influenced by molecule size and lipophilicity.

research LB1605 3D imaging of cleared ex vivo normal human skin, skin appendages and psoriasiform skin lesion using light-sheet microscopy

August 2018 in “Journal of Investigative Dermatology”

This study found that combining optical clearing methods with light-sheet fluorescence microscopy allows detailed 3D visualization of normal and pathological human skin biopsies, revealing differences in epidermal thickness and volume.

research Capturing Stem Cell Behavior Using Intravital and Live Cell Microscopy

10 citations , November 2019 in “Cold Spring Harbor Perspectives in Biology”

This review discusses how recent advances in live imaging have revealed the dynamic and adaptable nature of stem cells during various biological processes, and reports no clinical results.

research Scalp imaging techniques

5 citations , March 2017 in “Laser Physics Letters”

This review discusses the role of scalp imaging techniques in trichology for researching drug delivery and toxicology, without presenting new clinical results.

research Investigation of in vitro and in vivo skin permeability using laser scanning microscopy

January 2022 in “OPAL (Open@LaTrobe) (La Trobe University)”

This study used advanced optical imaging techniques to investigate nanoparticle and dye penetration in human skin, finding that nanoparticles mainly resided in the stratum corneum and accumulated in skin folds and hair follicles, while highlighting that sunscreen-based nanoparticles appear unlikely to pose safety concerns.