Long-Term Time-Lapse Multimodal Intravital Imaging of Regeneration and Bone Marrow-Derived Cell Dynamics in Skin

September 2013 in “ Technology ”

Ben K. Graf, Eric J. Chaney, Marina Marjanović, Steven G. Adie, Michael De Lisio, M. Carmen Valero, Marni D. Boppart, Stephen A. Boppart

skin regeneration bone marrow-derived cells collagen synthesis angiogenesis wound contraction TPEF SHG OCT GFP bone marrow transplanted mice cutaneous regeneration two-photon excitation fluorescence second harmonic generation optical coherence tomography green fluorescent protein

TLDR The study introduced a new imaging technology to track skin healing and bone marrow cell activity over time.

In the 2013 study, researchers introduced a new multimodal imaging technology combined with a non-rigid image registration method to observe and quantify skin regeneration and the dynamics of bone marrow-derived cells over extended periods. The technology utilized a combination of TPEF, SHG, and OCT imaging to visualize these processes in vivo in GFP bone marrow transplanted mice. The study successfully tracked various aspects of skin regeneration, such as collagen synthesis, angiogenesis, and wound contraction, over several months. The non-rigid registration algorithm was essential for accurately aligning images over time, considering the deformable nature of skin. This advancement provided significant insights into the role of bone marrow-derived cells in cutaneous regeneration and offered a valuable tool for future research in stem, immune, and tumor cell biology in vivo.

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Research cited in this study

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research Epidermal Homeostasis: A Balancing Act of Stem Cells in the Skin

1039 citations, February 2009 in “Nature Reviews Molecular Cell Biology”

Skin stem cells are crucial for maintaining and repairing the skin and hair, using a complex mix of signals to do so.

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