Multiphoton Microscopic Imaging of Rabbit Dorsal Skin

The study demonstrated that multiphoton microscopy (MPM) is an effective tool for high-resolution imaging of rabbit dorsal skin structures, such as keratin, collagen fibers, elastic fibers, and hair follicles, without the need for staining or fixation. MPM's noninvasive approach allows for the observation of skin components, which is useful for understanding skin physiology, pathology, and wound healing. The findings also indicated that rabbit skin contains fewer elastic fibers than human skin, potentially accounting for its lower elasticity, and that the organization of collagen bundles influences the intensity of imaging signals. The study highlights MPM's potential as a screening tool for medical and veterinary research, although the number of rabbits used was not specified.