Would You Recognize This Lesion as a Gumma Syphilitica?
June 2017
in “
Journal of The American Academy of Dermatology
”
LED light human outer root sheath cells hORSCs hair follicle hair growth MTT assay migration assay real-time PCR Western blot Wnt/ß-catenin signaling pathway ERK signaling pathway hair stem cell markers growth factors Wnt5a Axin2 Lef1 mRNA ß-catenin protein phosphorylation of ERK c-Jun p38 human dermal papilla cells hDPCs co-culture Wnt signaling pathway Lef1 ß-catenin ERK dermal papilla cells
TLDR LED light helps hair follicle cells grow and prevents them from dying by activating certain cell pathways.
The document presents a study investigating the effects of light-emitting diode (LED) light on human outer root sheath cells (hORSCs), which are important for hair follicle structure and hair growth. The study aimed to determine how different wavelengths of LED light affect hORSC proliferation, migration, and apoptosis. The researchers used MTT and migration assays to examine cell proliferation and migration, and real-time PCR and Western blot assays to analyze the expression of molecules related to the Wnt/ß-catenin and ERK signaling pathways, as well as hair stem cell markers and various growth factors. The results showed that low-level LED light irradiation promoted hORSC proliferation, suppressed apoptosis, and induced the expression of Wnt5a, Axin2, Lef1 mRNA, and ß-catenin protein. Additionally, phosphorylation of ERK, c-Jun, and p38 was observed, and LED-irradiated hORSCs increased the proliferation of human dermal papilla cells (hDPCs) in co-culture. The study concluded that LED light irradiation stimulates hORSC proliferation and migration, and inhibits apoptosis through the activation of the Wnt5a/ß-catenin and ERK signaling pathways. No commercial support was identified for this study.
