Knockout of Integrin Beta1 in Induced Pluripotent Stem Cells Accelerates Skin-Wound Healing by Promoting Cell Migration in Extracellular Matrix

July 2022 in “ Stem Cell Research & Therapy ”

Yu Ren, Zhibin Wang, Huijun Xu, Shun Wang, Shirui Li, Meng Xiang, SF Chen

integrin β1 Itgb1 induced pluripotent stem cells iPSCs extracellular matrix ECM wound healing angiogenesis blood perfusion cell migration ECM-crossing migration iPSC-ECM adhesion stem cells blood flow cell movement

TLDR Turning off a specific gene in stem cells speeds up skin healing by helping cells move better.

The study found that inhibiting the expression of integrin β1 (Itgb1) in induced pluripotent stem cells (iPSCs) can enhance their therapeutic potential for wound healing. The researchers knocked out Itgb1 in mouse iPSCs and found that this did not affect the cells' pluripotency, function, or survival, but it did accelerate wound healing, angiogenesis, blood perfusion, and survival in skin-wound lesions. The knockout also decreased iPSC-ECM adhesion while increasing ECM-crossing migration. The study suggests that inhibiting Itgb1 expression could be a strategy for increasing the efficacy of iPSC therapies.

View this study on stemcellres.biomedcentral.com →

Discuss this study in the Community →

Research cited in this study

9 / 9 results

AIRMESS – Academy of International Regenerative Medicine & Surgery Societies: Recommendations in the Use of Platelet-Rich Plasma (PRP) and Autologous Stem Cell-Based Therapy (ASC-BT) in Androgenetic Alopecia and Wound Healing

research AIRMESS – Academy of International Regenerative Medicine & Surgery Societies: Recommendations in the Use of Platelet-Rich Plasma (PRP) and Autologous Stem Cell-Based Therapy (ASC-BT) in Androgenetic Alopecia and Wound Healing

17 citations, March 2021 in “Expert Opinion on Biological Therapy”

Use PRP and ASC-BT for hair loss and wound healing, but more research needed.

Systematic Review: Adipose-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Biomaterials as New Regenerative Strategies in Chronic Skin Wounds and Soft Tissue Defects

research Systematic Review: Adipose-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Biomaterials as New Regenerative Strategies in Chronic Skin Wounds and Soft Tissue Defects

70 citations, February 2021 in “International Journal of Molecular Sciences”

Fat-derived stem cells, platelet-rich plasma, and biomaterials show promise for healing chronic skin wounds and improving soft tissue with few side effects.

Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil, Finasteride, and Adult Stem Cell-Based Therapy

research Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil, Finasteride, and Adult Stem Cell-Based Therapy

65 citations, April 2020 in “International Journal of Molecular Sciences”

PRP injections may be a safe, effective alternative for hair loss treatment compared to minoxidil and finasteride.

research Autologous Micrografts from Scalp Tissue: Trichoscopic and Long-Term Clinical Evaluation in Male and Female Androgenetic Alopecia

41 citations, January 2020 in “BioMed Research International”

Micrografts improve hair density and thickness without side effects.

Impact of Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma and Activated Platelet-Rich Plasma in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

research Impact of Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma and Activated Platelet-Rich Plasma in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

58 citations, January 2020 in “International Journal of Molecular Sciences”

Different methods of preparing Platelet-Rich Plasma (PRP) can affect wound healing and hair regrowth in plastic surgery. Using a kit with specific standards helps isolate PRP that meets quality criteria. Non-Activated PRP and Activated PRP have varying effects depending on the tissue and condition treated. For hair regrowth, Non-Activated PRP increased hair density more than Activated PRP. Both treatments improved various aspects of scalp health.

research Autologous Cellular Method Using Micrografts of Human Adipose Tissue Derived Follicle Stem Cells in Androgenic Alopecia

88 citations, July 2019 in “International Journal of Molecular Sciences”

Using human fat tissue derived stem cells in micrografts can safely and effectively increase hair density in people with hair loss.

Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development

research Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development

136 citations, May 2019 in “Cells”

Stem cell therapy, particularly using certain types of cells, shows promise for treating hair loss by stimulating hair growth and development, but more extensive trials are needed to confirm these findings.

Platelet-Rich Plasma and Micrografts Enriched with Autologous Human Follicle Mesenchymal Stem Cells Improve Hair Regrowth in Androgenetic Alopecia: Biomolecular Pathway Analysis and Clinical Evaluation

research Platelet-Rich Plasma and Micrografts Enriched with Autologous Human Follicle Mesenchymal Stem Cells Improve Hair Regrowth in Androgenetic Alopecia: Biomolecular Pathway Analysis and Clinical Evaluation

69 citations, April 2019 in “Biomedicines”

PRP and HF-MSCs treatment improves hair growth, thickness, and density in androgenetic alopecia.

Stem Cells from Human Hair Follicles: First Mechanical Isolation for Immediate Autologous Clinical Use in Androgenetic Alopecia and Hair Loss

research Stem Cells from Human Hair Follicles: First Mechanical Isolation for Immediate Autologous Clinical Use in Androgenetic Alopecia and Hair Loss

86 citations, June 2017 in “Stem cell investigation”

Stem cells from hair follicles can safely treat hair loss.

Similar Research

5 / 1000+ results

research Feasibility of Repairing Full-Thickness Skin Defects by iPSC-Derived Epithelial Stem Cells Seeded on a Human Acellular Amniotic Membrane

23 citations, May 2019 in “Stem cell research & therapy”

iPSC-derived stem cells on a special membrane can help repair full-thickness skin defects.

research The Developmental and Molecular Requirements for Ensuring That Human Pluripotent Stem Cell-Derived Hair Follicle Bulge Stem Cells Have Acquired Competence for Hair Follicle Generation Following Transplantation

4 citations, January 2021 in “Cell transplantation”

Scientists found the best time to transplant human stem cells for hair growth is between days 16-18 when they have the right markers and growth potential.

research The Secretome of Irradiated Peripheral Mononuclear Cells Attenuates Hypertrophic Skin Scarring

1 citations, March 2023 in “Pharmaceutics”

PBMCsec can help reduce and improve thick skin scars.

research Defining MicroRNA Signatures of Hair Follicular Stem and Progenitor Cells in Healthy and Androgenic Alopecia Patients

12 citations, November 2020 in “Journal of Dermatological Science”

Found microRNA differences in hair cells, suggesting potential treatment targets for hair loss.

research Targeting Integrin Pathways: Mechanisms and Advances in Therapy

54 citations, January 2023 in “Signal Transduction and Targeted Therapy”

New therapies are being developed that target integrin pathways to treat various diseases.