Knockout of Integrin Beta1 in Induced Pluripotent Stem Cells Accelerates Skin-Wound Healing by Promoting Cell Migration in Extracellular Matrix

July 2022 in “ Stem Cell Research & Therapy ”

Yu Ren, Zhibin Wang, Huijun Xu, Shun Wang, Shirui Li, Meng Xiang, SF Chen

integrin β1 Itgb1 induced pluripotent stem cells iPSCs extracellular matrix ECM wound healing angiogenesis blood perfusion cell migration ECM-crossing migration iPSC-ECM adhesion stem cells blood flow cell movement

TLDR Turning off a specific gene in stem cells speeds up skin healing by helping cells move better.

The study found that inhibiting the expression of integrin β1 (Itgb1) in induced pluripotent stem cells (iPSCs) can enhance their therapeutic potential for wound healing. The researchers knocked out Itgb1 in mouse iPSCs and found that this did not affect the cells' pluripotency, function, or survival, but it did accelerate wound healing, angiogenesis, blood perfusion, and survival in skin-wound lesions. The knockout also decreased iPSC-ECM adhesion while increasing ECM-crossing migration. The study suggests that inhibiting Itgb1 expression could be a strategy for increasing the efficacy of iPSC therapies.

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