Systematic Review: The Potential Implications of Different Platelet-Rich Plasma Concentrations in Regenerative Medicine for Tissue Repair

August 2020 in “ International Journal of Molecular Sciences ”

Pietro Gentile, Simone Garcovich

platelet-rich plasma PRP tissue regeneration growth factor diffusion

Studysummary This systematic review indicates that a platelet-rich plasma concentration of 1.0 × 10^6 plt/μL may be optimal for tissue regeneration due to sufficient nutrition supply and effective growth factor diffusion in vivo. Our plain-language summary of this paper — not a Tressless recommendation.

The systematic review from 2020 evaluated the effects of different platelet-rich plasma (PRP) concentrations on tissue repair, analyzing 15 studies from an initial 965 articles. It found that a PRP concentration of 1.0 × 10^6 plt/μL was optimal for tissue repair due to adequate capillary nutrition and growth factor diffusion. A PRP/media ratio of ≤10% (Vol/Vol) was ideal to prevent cellular starvation. The review highlighted the lack of standardized PRP preparation methods, which affected result consistency, and emphasized the need for standardized procedures to ensure reliable treatment outcomes. The review also noted that PRP's effectiveness varied by cell type and origin, with Human Mesenchymal Stem Cells being more responsive than fibroblasts.

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research Platelet-Rich Plasma and Its Use for Cicatricial and Non-Cicatricial Alopecias: A Narrative Review

12 citations , June 2020 in “Dermatology and therapy”

This article reviews major uses of platelet-rich plasma in treating various hair disorders, including both non-cicatricial and cicatricial alopecia, but reports no new results.

Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil, Finasteride, and Adult Stem Cell-Based Therapy

research Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil, Finasteride, and Adult Stem Cell-Based Therapy

65 citations , April 2020 in “International Journal of Molecular Sciences”

This review indicates that most studies reported positive effects of platelet-rich plasma injections for treating androgenic alopecia, with no major side effects, suggesting it may be a safe alternative to Minoxidil and Finasteride.

research Autologous Activated Platelet-Rich Plasma (AA-PRP) and Non-Activated (A-PRP) in Hair Growth: A Retrospective, Blinded, Randomized Evaluation in Androgenetic Alopecia

44 citations , February 2020 in “Expert Opinion on Biological Therapy”

In this study, autologous non-activated platelet-rich plasma was associated with greater hair re-growth than autologous activated platelet-rich plasma over long-term follow-up.

Impact of Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma and Activated Platelet-Rich Plasma in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

research Impact of Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma and Activated Platelet-Rich Plasma in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

58 citations , January 2020 in “International Journal of Molecular Sciences”

This review evaluates various kits for isolating platelet-rich plasma using established processing standards and finds they enable compliance with consensus quality criteria; ongoing research is needed to refine these procedures for improved consistency and safety.

research The Physician's Guide to Platelet-Rich Plasma in Dermatologic Surgery Part II: Clinical Evidence

24 citations , November 2019 in “Dermatologic Surgery”

This review evaluates the use of platelet-rich plasma in dermatology, highlighting favorable outcomes in several areas, especially androgenetic alopecia, despite a lack of large randomized controlled trials and consistency in protocols.

The Physician's Guide to Platelet-Rich Plasma in Dermatologic Surgery Part I: Definitions, Mechanisms of Action, and Technical Specifications

research The Physician's Guide to Platelet-Rich Plasma in Dermatologic Surgery Part I: Definitions, Mechanisms of Action, and Technical Specifications

16 citations , November 2019 in “Dermatologic Surgery”

This review examines the current evidence for platelet-rich plasma in dermatology, finding favorable outcomes particularly for androgenetic alopecia, but highlights the need for more rigorous clinical trials and standardized protocols.

research Autologous Cellular Method Using Micrografts of Human Adipose Tissue Derived Follicle Stem Cells in Androgenic Alopecia

88 citations , July 2019 in “International Journal of Molecular Sciences”

In this study of patients with Androgenic Alopecia, micro-grafts containing HD-AFSCs resulted in significantly improved hair density and follicle count over 11 months compared to a saline solution, suggesting a potential alternative therapy for hair regrowth.

research The Use of Platelet-Rich Plasma in Aesthetic and Regenerative Medicine: A Comprehensive Review

76 citations , December 2018 in “Aesthetic Plastic Surgery”

This review discusses various aesthetic and regenerative applications of platelet-rich plasma and concludes that it has potential as a safe cosmetic intervention but requires further studies to understand its clinical effects.

research Mechanical and Controlled PRP Injections in Patients Affected by Androgenetic Alopecia

January 2018 in “Journal of Visualized Experiments”

This study found that injections of autologous platelet-rich plasma in patients with pattern hair loss were compared to placebo, but the abstract does not report specific clinical results.

research Mechanical and Controlled PRP Injections in Patients Affected by Androgenetic Alopecia

24 citations , January 2018 in “Journal of Visualized Experiments”

In this study, 23 patients with androgenetic alopecia were treated with non-activated autologous platelet-rich plasma (A-PRP) administered through interfollicular injections, but the abstract provides no results of the treatment's effectiveness.

research Alopecia and Platelet-Derived Therapies

23 citations , November 2017 in “Stem cell investigation”

This study found that platelet lysate produced higher follicular regeneration in hair restoration surgeries compared to activated PRP and saline, with significant gains in both follicular unit and hair density.

Evaluation of Non-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems

research Evaluation of Non-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems

136 citations , February 2017 in “International Journal of Molecular Sciences”

This study observed that autologous platelet-rich plasma treatment improved hair count and density in areas with androgenetic alopecia compared to placebo, with differing outcomes based on the PRP collection device used.

research The Effect of Autologous Activated Platelet Rich Plasma Injection on Pattern Hair Loss: Clinical and Histomorphometric Evaluation

183 citations , January 2014 in “BioMed Research International”

This study reported that AA-PRP injections significantly increased hair count and density in individuals with pattern hair loss compared to baseline, demonstrating their potential for enhancing hair growth.