Impact of Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells and Human Adipose-Derived Mesenchymal Stem Cells: Enzymatic Digestion Versus Mechanical Centrifugation

The review examined the impact of different preparation methods for isolating human adipose-derived stromal vascular fraction cells (AD-SVFs) and mesenchymal stem cells (AD-MSCs), focusing on enzymatic digestion versus mechanical centrifugation. It highlighted the lack of standardized techniques and introduced processing quantitative standards (PQSs) and processing qualitative standards (PQLSs) to evaluate the procedures. PQSs involved variables like volume, time, and pressure, while PQLSs focused on the quality of materials and methods. The study found that using kits adhering to these standards could effectively isolate AD-MSCs meeting quality criteria. It suggested that as new critical quality attributes (CQAs) are discovered, these standards may need adjustments to improve reproducibility, quality, and safety, with further confirmatory studies required.