Impact of Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells and Human Adipose-Derived Mesenchymal Stem Cells: Enzymatic Digestion Versus Mechanical Centrifugation

    Pietro Gentile, Claudio Calabrese, Barbara De Angelis … Simone Garcovich
    Studysummary This review discusses different processing methods for isolating human AD-SVFs/AD-MSCs using various kits and reports no new clinical results, highlighting the need for standardized preparation techniques.
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    Research cited in this study 9

    1. Autologous Cellular Method Using Micrografts of Human Adipose Tissue Derived Follicle Stem Cells in Androgenic Alopecia International Journal of Molecular Sciences · 2019
    2. Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development Cells · 2019
    3. Platelet-Rich Plasma and Micrografts Enriched with Autologous Human Follicle Mesenchymal Stem Cells Improve Hair Regrowth in Androgenetic Alopecia: Biomolecular Pathway Analysis and Clinical Evaluation Biomedicines · 2019
    4. Mechanical and Controlled PRP Injections in Patients Affected by Androgenetic Alopecia Journal of Visualized Experiments · 2018
    5. Mechanical and Controlled PRP Injections in Patients Affected by Androgenetic Alopecia Journal of Visualized Experiments · 2018
    6. Alopecia and Platelet-Derived Therapies Stem cell investigation · 2017
    7. Stem Cells from Human Hair Follicles: First Mechanical Isolation for Immediate Autologous Clinical Use in Androgenetic Alopecia and Hair Loss Stem cell investigation · 2017
    8. Evaluation of Non-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems International Journal of Molecular Sciences · 2017
    9. The Effect of Platelet-Rich Plasma in Hair Regrowth: A Randomized Placebo-Controlled Trial Stem Cells Translational Medicine · 2015