Impact of Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells and Human Adipose-Derived Mesenchymal Stem Cells: Enzymatic Digestion Versus Mechanical Centrifugation

The document reviewed the impact of enzymatic digestion versus mechanical centrifugation methods for isolating human adipose-derived stromal vascular fraction cells (AD-SVFs) and mesenchymal stem cells (AD-MSCs). Enzymatic digestion generally yielded higher cell counts and viability, making it more effective for stem cell enrichment, while mechanical methods maintained tissue integrity and reduced contaminants. The study emphasized the need for standardized methods to ensure consistent cell isolation and suggested using modular kits to improve reproducibility. It also explored the potential of AD-MSCs in regenerative therapies, including hair loss treatment, and highlighted the importance of further research to enhance the safety and efficacy of these methods.