Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium

January 2016 in “ Methods in molecular biology ”

Mohsen Sagha, Nowruz Najafzadeh

CD34-positive hair-follicle-associated pluripotent stem cells retinoic acid serum-free medium magnetic-activated cell sorting neural-induction media reverse transcription polymerase chain reaction immunocytochemistry autologous stem cell therapy HAP stem cells MACS RT-PCR

TLDR Hair follicle stem cells can become nerve cells using specific treatments.

The study described the process of identifying and enriching CD34-positive hair-follicle-associated pluripotent (HAP) stem cells using magnetic-activated cell sorting (MACS) and inducing their differentiation into neuronal and glial cells with defined neural-induction media. The resulting neural cell populations were characterized through reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry assays. This research highlighted the potential of HAP stem cells as a promising autologous source for stem cell therapy, particularly in neural differentiation.

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