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Glossary Serum-Free Medium

growth medium without animal serum, using specific nutrients instead

Serum-Free Medium (SFM) is a type of growth medium used in cell culture that does not contain animal serum. Instead, it is supplemented with specific nutrients, hormones, and growth factors tailored to support the growth and maintenance of particular cell types. This allows for more controlled and reproducible experimental conditions, reducing variability and the risk of contamination from unknown serum components.

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Research

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research Prolonged maintenance of human hair follicles in vitro in a serum-free medium

36 citations , October 1993 in “British Journal of Dermatology”

In this study, isolated human hair follicles maintained prolonged growth and consistent DNA and keratin synthesis patterns in a fully defined medium for at least 9 days while preserving in vivo-like morphology.

research Organ culture of human hair follicles in serum-free medium

27 citations , January 1993 in “Archives of Dermatological Research”

In this study, minoxidil sulphate was observed to significantly increase DNA synthesis in cultured human hair follicles in a concentration-dependent manner.

research Murine Vibrissae Cultured in Serum-Free Medium Reinitiate Anagen

13 citations , August 2007 in “Journal of Investigative Dermatology”

Mouse hair can regrow in a special lab setup without serum.

research Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium

4 citations , January 2016 in “Methods in molecular biology”

This chapter reviews the methodology for isolating and differentiating CD34+ HAP stem cells into neural cells, but provides no new research results.

DermaCult Keratinocyte Expansion Medium: A Serum- and BPE-Free Medium Supporting Long-Term Feeder-Free Expansion of Primary Human Epidermal and Pluripotent Stem Cell-Derived Keratinocytes

research 566 DermaCult™ Keratinocyte Expansion Medium, a Serum- and BPE-free Medium Supporting Long-term Feeder-free Expansion of Primary Human Epidermal and Pluripotent Stem Cell-Derived Keratinocytes

November 2022 in “Journal of Investigative Dermatology”

This study found that DermaCult™ Keratinocyte Expansion Medium allows for significantly extended growth of human epidermal keratinocytes while maintaining their differentiation potential.

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Community Join

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community DHT Itch and Oily Scalp Explained (and how to fix it)

90 upvotes 3 months ago

Malassezia overgrowth can worsen oily scalp and inflammation, contributing to hair loss. Treatments include antifungal shampoos, C6/C8 MCT oil, low-dose isotretinoin, finasteride, and dutasteride.

community Microneedling aftercare advice

4 upvotes 4 years ago

Microneedling aftercare involves using saline water instead of sea water, and avoiding hyaluronic acid due to its potential pro-inflammatory effects. Users suggest using jojoba oil as a carrier for essential oils like rosemary and lavender, and combining microneedling with minoxidil for better hair regrowth results.

community Had a hormone panel to determine if I’m in range to use finasteride without too many issues. Thoughts?

5 upvotes 3 years ago

A user shared their hormone levels to gauge if they can use finasteride for hair loss without issues. Their hormone levels are mostly within the reference ranges.

community Is it worth using anti-hair loss shampoo?

in Product 2 upvotes 7 months ago

Anti-hair loss shampoos, like the one with rosemary, quillaja, and jaborandi extracts, mainly support scalp health but are not effective for hair regrowth compared to treatments like finasteride or minoxidil. Ketoconazole 2% is noted as the only effective anti-hair loss shampoo, while others are often considered scams.

community Creatine Does Not Increase DHT And Does Not Cause Hair Loss - Randomized Controlled Trial

308 upvotes 1 year ago

Creatine does not increase DHT levels or cause hair loss. Personal experiences vary, but scientific evidence shows no link between creatine and hair loss.

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Clínica SerumMérida, Yucatán · MX

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