Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering
January 2019
in “
Methods in molecular biology
”
Studysummary This chapter describes methods for creating engineered skin substitutes with chimeric hair follicles using human and murine cell grafts in immunodeficient mice, but reports no clinical outcomes.
Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer
The document described methods for creating engineered skin substitutes that incorporated chimeric hair follicles, aiming to improve treatments for full-thickness burns and other skin injuries. The process involved isolating human keratinocytes, human fibroblasts, and murine dermal papilla cells, which were then combined with collagen-glycosaminoglycan scaffolds to produce human-murine chimeric grafts. These grafts were applied to full-thickness surgical wounds in immunodeficient mice, resulting in the formation of a human-mouse follicular structure. This approach aimed to enhance the functionality of skin substitutes by integrating essential skin appendages like hair follicles.
