Assessment of Replication Rates of Human Keratinocytes in Engineered Skin Substitutes Grafted to Athymic Mice

June 2012 in “ Wound Repair and Regeneration ”

Steven T. Boyce, Rachel K. Rice, Kaari A. Lynch, Andrew P. Supp, Viki B. Swope, Richard J. Kagan, Dorothy M. Supp

human keratinocytes engineered skin substitutes athymic mice 5-bromo-2'-deoxyuridine BrdU basal keratinocytes in vitro in vivo proliferative phenotype wound healing keratinocytes ESS nude mice lab mice cell replication skin grafts

TLDR Regulating keratinocyte growth in engineered skin can improve wound healing.

The study assessed the replication rates of human keratinocytes in engineered skin substitutes (ESS) grafted to athymic mice. Using 5-bromo-2'-deoxyuridine (BrdU) labeling, it was found that basal keratinocytes in ESS showed differential rates of label uptake and retention both in vitro and in vivo. Label uptake decreased gradually over time, indicating a proliferative phenotype with potential for long-term replication. This suggests that regulating the proliferative phenotype in keratinocytes of ESS could enhance the biological similarity of engineered skin to natural skin and improve wound healing stability.

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