Development of Pigmented Reconstructed Human Epidermis Model Containing Human Melanoblasts from Keratinocyte Culture

The study detailed the creation of a pigmented reconstructed human epidermis model that incorporated human melanoblasts obtained from keratinocyte cultures. Researchers used a 'calcium pulse' method to isolate melanoblasts, which led to an increased number of melanocytic cells. The models with melanoblasts demonstrated enhanced proliferation and a well-differentiated epidermis compared to those with only melanocytes. The study concluded that these models are useful for studying skin pigmentation and can be used as an alternative to animal testing.