Culture of Amelanotic Melanocytes Derived from Human Fetal Hair Follicles
June 2013
in “
In Vitro Cellular & Developmental Biology - Animal
”
TLDR Melanocytes from human fetal hair follicles were successfully cultured, showing potential for hair disease research and clinical use.
The study successfully cultured melanocytes derived from human fetal hair follicles without using a feeder layer, indicating the medium was suitable for the growth of melanocyte stem cells (MSCs) and their progenies. These cultured melanocytes exhibited a bipolar or tripolar appearance, differing from epidermal melanocytes, and showed high proliferative capability and clonal growth behavior. Immunocytochemical staining and Fontana Masson staining confirmed the presence of melanocytes, and atomic force microscopy revealed distinct morphological features such as rounded, oval, triangular, or quadrangular perikarya with dendritic structures. This research provided insights into the potential for developing cell lines from human hair follicles to better understand hair-related diseases and meet clinical needs.
