The Empowering Influence of Air-Liquid Interface Culture on Skin Organoid Hair Follicle Development

This study demonstrates that the air-liquid interface (ALI) culture method significantly enhances the development and maturation of hair follicles in skin organoids derived from human-induced pluripotent stem cells. Compared to conventional floating culture, the ALI model, which uses transwell membranes, results in skin organoids with more numerous and better-developed hair follicles, as well as the formation of sweat and sebaceous glands and pigmentation. The findings suggest that ALI culture could improve the efficiency and effectiveness of skin organoid development, with potential applications in regenerative medicine and hair follicle regeneration studies. However, challenges such as off-target tissue formation and the absence of critical cell populations remain.