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New methods for growing skin cells can improve skin grafts by building blood vessels within them.

This study supports that an antibody response against anagen-specific hair follicle antigens, including trichohyalin and keratin 16, may play a role in alopecia areata.

This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

This review study focused on various in vitro methods to evaluate skin permeation and retention of sunscreens, highlighting the importance of assessing sunscreen penetration due to widespread nanomaterial usage and advancements in cosmetic formulation.

This study introduced a simple and reliable in vitro assay capable of large-scale simultaneous screening of hair growth-promoting compounds using a 3D co-culture system of human dermal papilla and outer root sheath cells.

This study successfully improved methods for isolating, culturing, and purifying rat hair follicle stem cells, demonstrating their high purity, strong proliferation, and potential for adipogenic and osteogenic differentiation in vitro, which could support advancements in tissue engineering.

This study developed a method to culture and maintain chicken feather follicles in vitro, preserving structure and biology similar to their in vivo state, though some gene expression was altered.

This study describes a new tissue culture method that successfully induces outer root sheath cells from hair follicles to differentiate into a multilayered epithelium with characteristics similar to normal interfollicular epidermis.

This review covers potential molecular targets for androgenetic alopecia treatments but presents no new experimental findings; the authors highlight various in vitro and in vivo screening methods for therapeutic compounds.

This study investigated the effects of substance P on human hair growth but found its role in hair follicle physiology remains unclear.

This study established an optimal in vitro culture system for fine-wool sheep hair follicle stem cells, finding a combination of two-step enzymatic digestion and mechanical separation to be the most effective method.

This study found that a novel 3D in vitro culture condition improved stemness markers and proliferation potential in human dermal stem/progenitor cells compared to traditional 2D methods.

Different lab conditions and light treatment methods change how human skin cells respond to light therapy.

This study found that epidermal stem cells are located in rats' hair follicle bulge regions, and the methods used to isolate and culture these cells in vitro are feasible.

This study concluded that high-purity rat hair follicle stem cells with strong proliferation abilities can be obtained using a modified culture and purification method.

This article reviews in vitro methods for evaluating whitening, anti-wrinkle, and hair growth ingredients in cosmetics, noting that more human-relevant evaluation methods with reliable biomarkers are needed; it presents no new clinical outcomes.

This review introduces in vitro evaluation methods for whitening, anti-wrinkle, and hair growth cosmetic ingredients but reports no new experimental results, highlighting the need for better human-correlated methods and reliable biomarkers.

This study developed a method for expanding human dermal papilla cells in vitro while maintaining their ability to induce hair growth, advancing the potential for follicular cell implantation as a treatment for hair loss.

In this study, mouse hair follicle stem cells cultured in mouse embryonic fibroblast/keratinocyte serum-free medium exhibited the highest proliferative ability and stronger osteogenic differentiation potential, similar to hair follicle dermal papilla cells.

This study demonstrated that using a perfusion culture bioreactor and 3D spheroid culture can enhance corneal endothelial cell expansion and support the construction of tissue-engineered corneal endothelial layers in vitro.

This review suggests that omitting the proteolysis step and including tissue pieces in mesenchymal stem cell isolation may improve cell quality, reduce contamination risk, and lower costs and processing time in clinical applications.

Minoxidil enhances hair growth and preserves the root sheath in cultured follicles.

This study found that culturing hair follicles at the air-liquid interface enhanced hair growth and maintained an anagen-like morphology better than submerged cultures.

This study identified highly variable genes in mesenchymal stem/stromal cells that are linked to classic functions like development and inflammation response, suggesting their potential as markers for further potency studies.

This article discusses methods to evaluate hair growth stimulants and reports no new clinical results; the authors highlight the need for more precise identification of stem cell sites in hair follicles.

This study describes a protocol for generating hair follicle germ-like organoids from human skin cells, which may aid in alopecia drug discovery and understanding hair follicle regeneration.

This study developed in vitro methods to regenerate hair follicles and erector muscles by mimicking their native structures and mechanical conditions, reconstructing hair follicle-like structures and forming muscle-like tissues.

This study introduces a method for long-term expansion of adult mouse epidermal stem cells in vitro using an organoid culture system that maintains the basal-apical organization.

This chapter reviews surgical management techniques for leukotrichia in vitiligo and presents no new clinical findings; it highlights various grafting and epilation methods.

Cultured hair follicles need careful handling and respond well to growth factors.