Explant Culture: An Advantageous Method for Isolation of Mesenchymal Stem Cells from Human Tissues

    February 2017 in “ Cell Proliferation ”
    Fatemeh Hendijani
    Studysummary This review suggests that omitting the proteolysis step and including tissue pieces in mesenchymal stem cell isolation may improve cell quality, reduce contamination risk, and lower costs and processing time in clinical applications.
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    Research cited in this study 9

    1. Explant Culture: An Advantageous Method for Isolation of Mesenchymal Stem Cells from Human Tissues Cell Proliferation · 2017
    2. Induced Pluripotent Stem Cells From Human Hair Follicle Mesenchymal Stem Cells Stem Cell Reviews and Reports · 2012
    3. Human Epidermal Neural Crest Stem Cells (hEPI-NCSC): Characterization and Directed Differentiation into Osteocytes and Melanocytes Stem Cell Reviews and Reports · 2011
    4. The Mesenchymal Component of Hair Follicle Neogenesis: Background, Methods, and Molecular Characterization Experimental Dermatology · 2009
    5. The Hair Cycle Journal of Cell Science · 2006
    6. Simple and Rapid Method to Isolate and Culture Follicular Papillae from Human Scalp Hair Follicles Experimental Dermatology · 2002
    7. Expression of Vascular Endothelial Growth Factor (VEGF) in Various Compartments of the Human Hair Follicle Archives of Dermatological Research · 1998
    8. Growth Factor and Growth Factor Receptor Localization in the Hair Follicle Bulge and Associated Tissue in Human Fetus Journal of Investigative Dermatology · 1996
    9. Hair Growth Induction: Roles of Growth Factors Annals of the New York Academy of Sciences · 1991