ROCK Inhibitor Y-27632 Maintains the Propagation and Characteristics of Hair Follicle Stem Cells

January 2018 in “ PubMed ”

Liyou An, Pingping Ling, Jing Cui, Jiqiang Wang, Xiumei Zhu, Jiao Liu, Yujian Dai, Yanhong Liu, Lan Yang, Fuliang Du

ROCK inhibitor Y-27632 hair follicle stem cells HFSCs CD34 K15 ITGB1 keratinocytes adipose cells epidermal growth factor

TLDR Y-27632 helps hair follicle stem cells grow and keep their abilities.

The study showed that the ROCK inhibitor Y-27632 effectively preserved the propagation and characteristics of hair follicle stem cells (HFSCs), maintaining key markers and a stable ratio over multiple passages. In contrast, HFSCs cultured with epidermal growth factor lost these characteristics. Y-27632-derived HFSCs could form spherical clusters, differentiate into keratinocytes and adipose cells, and regenerate hair follicles in vivo when combined with fetal dermal cells in immune-deficient mice. These results indicated that Y-27632 maintained the self-renewal and differentiation potential of HFSCs, highlighting their potential for tissue engineering and clinical applications.

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