Proteomics Characterization of Primary Human Oral Epithelial Cells Using a Novel Culture Technique for Tissue Regeneration
August 2015
in “
MOJ proteomics & bioinformatics
”
oral mucosa keratinocytes Epithelial-derived Pop-Up Keratinocytes ePUKs DKK1 SERPINE1 follistatin tenascin-C hair follicle development stem cell niche maintenance tissue-engineered oral mucosa EVPOME wound healing keratinocytes pop-up keratinocytes dickkopf-related protein 1 plasminogen activator inhibitor-1 stem cell niche engineered oral mucosa
TLDR ePUKs could be valuable for regenerative medicine due to their wound healing abilities.
The study characterized a unique population of undifferentiated oral mucosa keratinocytes, termed "Epithelial-derived Pop-Up Keratinocytes (ePUKs)," using Gravity Assisted Cell Sorting (GACS) and LC/MS/MS analysis. These ePUKs exhibited increased expression of proteins such as DKK1, SERPINE1, follistatin, and tenascin-C, which are involved in cellular movement, hair follicle development, and stem cell niche maintenance. The use of ePUKs in fabricating tissue-engineered oral mucosa (EVPOME) showed enhanced protein abundance, suggesting that ePUKs could be a valuable cell source for regenerative medicine due to their wound healing promotion capabilities.
