PBX1-SIRT1 Positive Feedback Loop Attenuates ROS-Mediated HF-MSC Senescence and Apoptosis
April 2022
in “
Research Square (Research Square)
”
TLDR PBX1 reduces aging and cell death in stem cells by boosting SIRT1 and lowering PARP1.
The study demonstrates that PBX1 overexpression reduces hair follicle-derived mesenchymal stem cell (HF-MSC) senescence and apoptosis by upregulating SIRT1 and downregulating PARP1, leading to increased NAD and ATP levels. Conversely, SIRT1 knockdown or PARP1 overexpression exacerbates senescence and apoptosis through increased ROS and DNA damage. PBX1 can counteract the negative effects of SIRT1 knockdown or PARP1 overexpression. The findings reveal a positive feedback loop between PBX1 and SIRT1, positioning PBX1 upstream of SIRT1, and highlight the PBX1-SIRT1-PARP1 axis as crucial in mitigating HF-MSC senescence and apoptosis, offering insights into stem cell aging and potential therapeutic strategies for age-related diseases.
