PBX1-SIRT1 Positive Feedback Loop Attenuates ROS-Mediated HF-MSC Senescence and Apoptosis

August 2022 in “ Stem cell reviews and reports ”

Yuan Wang, Yutong Sui, Ye Niu, Dan Liu, Qi Xu, Feilin Liu, Kuiyang Zuo, Mingsheng Liu, Weiyong Sun, Ziyu Wang, Zinan Liu, Fei Zou, Jiahong Shi, Xiaomei Liu, Jinyu Liu

PBX1 SIRT1 PARP1 hair follicle-derived mesenchymal stem cells HF-MSCs senescence apoptosis NAD ATP reactive oxygen species ROS DNA damage hair follicle stem cells stem cell aging cell death energy molecules oxidative stress

Studysummary This study found that PBX1 overexpression reduces hair follicle-derived mesenchymal stem cell senescence and apoptosis by interacting with SIRT1 and PARP1, highlighting a potential mechanism for addressing aging-related diseases.

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The study investigated the relationship between pre-B-cell leukemia transcription factor1 (PBX1), Silent Information Regulator 1 (SIRT1), and Poly (ADP-ribose) polymerase-1 (PARP1) in the context of hair follicle-derived mesenchymal stem cells (HF-MSCs) senescence and apoptosis. The researchers found that overexpression of PBX1 reduced senescence and apoptosis in HF-MSCs, which was associated with an increase in SIRT1 and a decrease in PARP1, as well as higher levels of intracellular NAD and ATP. Conversely, knocking down SIRT1 led to increased senescence and apoptosis, higher reactive oxygen species (ROS) accumulation, and DNA damage, along with reduced NAD and ATP levels. However, overexpressing PBX1 could rescue the negative effects of SIRT1 knockdown. The study also showed that PBX1 could counteract the ATP and NAD depletion caused by PARP1 overexpression by increasing SIRT1 expression. These findings suggest the existence of a positive feedback loop between PBX1 and SIRT1 that plays a crucial role in mitigating HF-MSCs senescence and apoptosis, offering new insights into stem cell aging and potential strategies for age-related disease prevention and treatment.

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PBX1 Attenuates Hair Follicle-Derived Mesenchymal Stem Cell Senescence and Apoptosis by Alleviating Reactive Oxygen Species-Mediated DNA Damage Instead of Enhancing DNA Damage Repair

research PBX1 Attenuates Hair Follicle-Derived Mesenchymal Stem Cell Senescence and Apoptosis by Alleviating Reactive Oxygen Species-Mediated DNA Damage Instead of Enhancing DNA Damage Repair

9 citations , November 2021 in “Frontiers in Cell and Developmental Biology”

This study found that PBX1 overexpression reduces hair follicle-derived mesenchymal stem cell senescence and apoptosis by alleviating ROS-mediated DNA damage, rather than enhancing DNA repair.

PBX Homeobox 1 Enhances Hair Follicle Mesenchymal Stem Cell Proliferation and Reprogramming Through Activation of the AKT/Glycogen Synthase Kinase Signaling Pathway and Suppression of Apoptosis

research PBX Homeobox 1 Enhances Hair Follicle Mesenchymal Stem Cell Proliferation and Reprogramming Through Activation of the AKT/Glycogen Synthase Kinase Signaling Pathway and Suppression of Apoptosis

26 citations , August 2019 in “Stem Cell Research & Therapy”

This study found that PBX1 enhances the proliferation and reprogramming of hair follicle mesenchymal stem cells by activating the AKT/GSK3β signaling pathway, promoting NANOG expression, and inhibiting apoptosis.