Isolation of Stem Cells and Progenitors from Mouse Epidermis

May 2017 in “ Current Protocols in Stem Cell Biology ”

Lana Kostic, Egor Sedov, Despina Soteriou, Yahav Yosefzon, Yaron Fuchs

hair follicle stem cells epidermal progenitors fluorescence activated cell-sorting CD34 Keratin 15 bulge region in vivo models in vitro models HFSCs FACS K15

TLDR A method was developed to isolate and study hair follicle stem cells from mouse skin.

The document described a method for isolating hair follicle stem cells (HFSCs) and epidermal progenitors from mouse skin using fluorescence activated cell-sorting (FACS). The study focused on the distinct compartments of the epidermis, including the interfollicular epidermis, sweat glands, sebaceous glands, and hair follicles, highlighting the cyclical nature of hair follicle growth. HFSCs, located in the bulge niche and expressing markers like CD34 and Keratin 15, were isolated for further expansion and use in various in vivo and in vitro models to study their functions.

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