Isolation of Stem Cells and Progenitors from Mouse Epidermis

May 2017 in “ Current Protocols in Stem Cell Biology ”

Lana Kostic, Egor Sedov, Despina Soteriou, Yahav Yosefzon, Yaron Fuchs

hair follicle stem cells epidermal progenitors fluorescence activated cell-sorting CD34 Keratin 15 bulge region in vivo models in vitro models HFSCs FACS K15

TLDR A method was developed to isolate and study hair follicle stem cells from mouse skin.

The document described a method for isolating hair follicle stem cells (HFSCs) and epidermal progenitors from mouse skin using fluorescence activated cell-sorting (FACS). These HFSCs, located in the bulge region of the hair follicle, expressed markers such as CD34 and Keratin 15 (K15), which facilitated their isolation. Once isolated, these cells could be expanded and used in various in vivo and in vitro models to study their functions. This method provided a powerful tool for researching the unique properties and roles of HFSCs in the epidermis.

View this study on currentprotocols.onlinelibrary.wiley.com →

Discuss this study in the Community →

Research cited in this study

14 / 14 results

research Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

23 citations , April 2016 in “Journal of Visualized Experiments”

The method successfully isolates hair follicle stem cells from mice for research.

research Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

13 citations , April 2016 in “Journal of Visualized Experiments”

The method successfully isolates hair follicle stem cells and skin cells from mice for research.

research Wound Healing and Skin Regeneration

359 citations , January 2015 in “Cold Spring Harbor Perspectives in Medicine”

Hair growth phase and certain genes can speed up wound healing, while an inflammatory mediator can slow down new hair growth after a wound. Understanding these factors can improve tissue regeneration during wound healing.

research Stem Cell Dynamics in the Hair Follicle Niche

135 citations , December 2013 in “Seminars in Cell & Developmental Biology”

Stem cells in the hair follicle are regulated by their surrounding environment, which is important for hair growth.

research Sept4/ARTS Regulates Stem Cell Apoptosis and Skin Regeneration

78 citations , June 2013 in “Science”

Mice without the Sept4/ARTS gene heal wounds better due to more stem cells that don't die easily.

research Identification of Stem Cell Populations in Sweat Glands and Ducts Reveals Roles in Homeostasis and Wound Repair

265 citations , July 2012 in “Cell”

The study found that sweat glands contain different types of stem cells that help with healing and maintaining healthy skin.

research Anatomy and Physiology of the Skin

277 citations , July 2011 in “Journal of the Dermatology Nurses’ Association”

The skin's layers protect, sense, and regulate the body's internal balance, but can be prone to cancer.

research Lgr5 Marks Cycling, Yet Long-Lived, Hair Follicle Stem Cells

835 citations , October 2008 in “Nature Genetics”

Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

research Human Skin Stem Cells and the Aging Process

143 citations , September 2008 in “Experimental gerontology”

Skin aging is due to impaired stem cell mobilization or fewer responsive stem cells.

research Scratching the Surface of Skin Development

788 citations , February 2007 in “Nature”

The document concludes that skin stem cells are important for hair growth and wound healing, and could be used in regenerative medicine.

research The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells

207 citations , July 2006 in “Development”

MTS24 marks a new type of skin cell that helps hair growth and repair.

research The Hair Cycle

375 citations , February 2006 in “Journal of Cell Science”

The document concludes that the hair cycle is a complex process involving growth, regression, and rest phases, regulated by various molecular signals.

research Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis

1279 citations , November 2005 in “Nature Medicine”

research Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34

561 citations , April 2003 in “Journal of Investigative Dermatology”

Related Community Posts Join

6 / 1000+ results

community New and Interesting HairLoss Studies/Papers/Reviews

in Treatment 44 upvotes 5 years ago

Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

community The Real Cause Of Androgenetic Alopecia

in Research/Science 682 upvotes 1 year ago

Androgenetic alopecia is caused by DHT affecting hair growth. Finasteride and minoxidil are used to manage hair loss by blocking DHT and promoting hair growth.

Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

in Research 865 upvotes 1 year ago

The treatment for androgenetic alopecia involves using finasteride and minoxidil with intense exercise and cold exposure to boost metabolism and reduce androgenic effects, potentially leading to hair regrowth. This approach may activate biological pathways for improved hair and overall health.

community Confused why people are calling PP405 a cure...it never claimed to be able to undo miniaturization or revert vellus back to terminal hairs. It seems to be more of a growth stimulant like minoxidil.

in Research/Science 53 upvotes 10 months ago

PP405 is not a cure for hair loss but may reactivate dormant hair follicles, similar to minoxidil. It is unlikely to help with miniaturized or vellus hairs and is still in trial phases, with availability expected around 2030.

community PP405: The Ultimate Hair Loss Drug for Complete Hair Growth

in Research/Science 210 upvotes 10 months ago

PP405 is a new hair loss treatment in phase 2 trials that may promote hair growth by increasing lactate production and activating hair follicle stem cells. It could potentially replace hormone-disrupting treatments like Minoxidil and finasteride.

community The end of balding: We may have just found the secret to hair regrowth

in Technology 676 upvotes 6 months ago

MCL-1 is important for hair follicle stem cell survival, but its impact on human hair regrowth is unclear. Minoxidil and finasteride are the main treatments, with doubts about new discoveries leading to effective human solutions soon.

Related Research

7 / 7 results

research Isolation of Stem Cells and Progenitors from Mouse Epidermis

13 citations , May 2017 in “Current Protocols in Stem Cell Biology”

A method was developed to isolate and study hair follicle stem cells from mouse skin.

research Damage of Hair Follicle Stem Cells and Alteration of Keratin Expression in External Radiation-Induced Acute Alopecia

28 citations , June 2012 in “International Journal of Molecular Medicine”

Radiation damages hair stem cells and changes keratin expression, with Krt5 as a potential marker for radiation effects.

research Epidermal Stem Cells: Practical Perspectives and Potential Uses

62 citations , April 2009 in “British Journal of Dermatology”

Epidermal stem cells could lead to new treatments for skin and hair disorders.

research The up‐regulation of 14‐3‐3 proteins in Smad4 deficient epidermis and hair follicles at catagen

14 citations , April 2008 in “PROTEOMICS”

Increased 14-3-3 proteins may block hair cycle regression, causing hair loss.

research Manipulation of stem cell proliferation and lineage commitment:visualisation of label-retaining cells in wholemounts of mouse epidermis

421 citations , September 2003 in “Development”

Stem cell behavior varies with stimuli, and lineage changes can happen without affecting stem cell division.

research Expression of ΔNLef1 in mouse epidermis results in differentiation of hair follicles into squamous epidermal cysts and formation of skin tumours

297 citations , January 2002 in “Development”

Overexpression of ΔNLef1 in mouse skin leads to hair loss, cysts, and skin tumors.

research Genetically Null Mice Reveal a Central Role for Epidermal Growth Factor Receptor in the Differentiation of the Hair Follicle and Normal Hair Development

107 citations , June 1997 in “PubMed”

EGFR is essential for normal hair development and follicle differentiation.