Isolation of Stem Cells and Progenitors from Mouse Epidermis

May 2017 in “ Current Protocols in Stem Cell Biology ”

Lana Kostic, Egor Sedov, Despina Soteriou, Yahav Yosefzon, Yaron Fuchs

hair follicle stem cells epidermal progenitors fluorescence activated cell-sorting CD34 Keratin 15 bulge region in vivo models in vitro models HFSCs FACS K15

Studysummary This study outlines a method to isolate hair follicle stem cells from mouse skin using fluorescence activated cell-sorting for use in research models, but reports no experimental findings. Our plain-language summary of this paper — not a Tressless recommendation.

The document described a method for isolating hair follicle stem cells (HFSCs) and epidermal progenitors from mouse skin using fluorescence activated cell-sorting (FACS). The study focused on the distinct compartments of the epidermis, including the interfollicular epidermis, sweat glands, sebaceous glands, and hair follicles, highlighting the cyclical nature of hair follicle growth. HFSCs, located in the bulge niche and expressing markers like CD34 and Keratin 15, were isolated for further expansion and use in various in vivo and in vitro models to study their functions.

View this study on currentprotocols.onlinelibrary.wiley.com →

Discuss this study in the Community →

Research cited in this study

16 / 16 results

research Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

13 citations , April 2016 in “Journal of Visualized Experiments”

This study describes a method for isolating stem cells and keratinocytes from mouse hair follicles using FACS technology, facilitating the study of stem cell biology.

research Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

23 citations , April 2016 in “Journal of Visualized Experiments”

This research details a robust method for isolating hair follicle stem cells and epidermal keratinocytes from mouse hair follicles using fluorescence activated cell-sorting technology.

research Wound Healing and Skin Regeneration

359 citations , January 2015 in “Cold Spring Harbor Perspectives in Medicine”

This article reviews the role of skin appendages in wound healing and appendage regeneration, discussing cellular and molecular mechanisms but reports no new experimental findings.

research Stem Cell Dynamics in the Hair Follicle Niche

135 citations , December 2013 in “Seminars in Cell & Developmental Biology”

This review examines the literature on the molecular and cellular makeup of the hair follicle niche and how it influences stem cell behavior during hair regeneration, but does not provide new experimental results.

research Sept4/ARTS Regulates Stem Cell Apoptosis and Skin Regeneration

78 citations , June 2013 in “Science”

This study found that mice lacking the Sept4/ARTS gene had more hair follicle stem cells and improved wound healing, suggesting apoptosis regulation as a potential target in regenerative medicine.

research Identification of Stem Cell Populations in Sweat Glands and Ducts Reveals Roles in Homeostasis and Wound Repair

265 citations , July 2012 in “Cell”

This study found multipotent progenitors in sweat ducts that become unipotent after sweat gland development, highlighting distinct regenerative capabilities in adult glandular skin stem cell populations.

research Anatomy and Physiology of the Skin

277 citations , July 2011 in “Journal of the Dermatology Nurses’ Association”

The skin's layers protect, sense, and regulate the body's internal balance, but can be prone to cancer.

research Lrig1 Expression Defines a Distinct Multipotent Stem Cell Population in Mammalian Epidermis

503 citations , May 2009 in “Cell stem cell”

This study suggests that Lrig1-positive cells form a previously unknown reservoir of interfollicular epidermal stem cells in adult mouse skin, influencing epidermal proliferation and hair follicle development.

research Lgr5 Marks Cycling, Yet Long-Lived, Hair Follicle Stem Cells

835 citations , October 2008 in “Nature Genetics”

Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

research Human Skin Stem Cells and the Aging Process

143 citations , September 2008 in “Experimental gerontology”

This review discusses the diverse roles and characteristics of epidermal and dermal stem cells in skin regeneration and aging, highlighting their potential applications in regenerative medicine and reports no new clinical results.

research Nfatc1 Balances Quiescence and Proliferation of Skin Stem Cells

415 citations , January 2008 in “Cell”

NFATc1 controls hair stem cell activity, affecting hair growth and could be a target for hair loss treatments.

research Scratching the Surface of Skin Development

788 citations , February 2007 in “Nature”

This review explores how adult skin epithelia preserve stem cell populations for hair follicle regeneration and wound healing, but reports no new experimental results.

research The Cell-Surface Marker MTS24 Identifies a Novel Population of Follicular Keratinocytes With Characteristics of Progenitor Cells

207 citations , July 2006 in “Development”

This study identified a novel MTS24-positive murine keratinocyte population with increased colony-forming ability, suggesting these cells may serve as a new reservoir of proliferative progenitor or stem cells in hair follicles.

research The Hair Cycle

375 citations , February 2006 in “Journal of Cell Science”

This article reviews the stages and regulation of the hair cycle, highlighting molecular regulators involved, but it does not present new research findings.

research Stem Cells in the Hair Follicle Bulge Contribute to Wound Repair but Not to Homeostasis of the Epidermis

1279 citations , November 2005 in “Nature Medicine”

research Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34

561 citations , April 2003 in “Journal of Investigative Dermatology”

CD34 is a marker for isolating stem-like cells in mouse hair follicles.