Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

April 2016 in “ Journal of Visualized Experiments ”

Despina Soteriou, Lana Kostic, Egor Sedov, Yahav Yosefzon, Hermann Steller, Yaron Fuchs

hair follicle stem cells epidermal keratinocytes fluorescence activated cell-sorting CD34 keratin 15 bulge region multipotency quiescence activation FACS K15

TLDR The method successfully isolates hair follicle stem cells from mice for research.

The document described a protocol for isolating hair follicle stem cells (HFSCs) and epidermal keratinocytes from the dorsal skin of adult mice. This method utilized fluorescence activated cell-sorting (FACS) technology to enrich and isolate these cell populations, specifically using markers like CD34 for HFSCs and Sca-1 for epidermal keratinocytes. The protocol was noted for its speed and reliability, allowing for the selection and harvesting of specific cell types from heterogeneous populations. This approach was advantageous over other methods, such as tissue dissociation, and could be applied to isolate other cell populations within the hair follicle structure.

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Research cited in this study

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research Sept4/ARTS Regulates Stem Cell Apoptosis and Skin Regeneration

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research Epidermal Homeostasis: A Balancing Act of Stem Cells in the Skin

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research Lgr5 Marks Cycling, Yet Long-Lived, Hair Follicle Stem Cells

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Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

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research The Cell-Surface Marker MTS24 Identifies a Novel Population of Follicular Keratinocytes With Characteristics of Progenitor Cells

207 citations , July 2006 in “Development”

MTS24 marks a new type of skin cell that helps hair growth and repair.

research The Hair Cycle

375 citations , February 2006 in “Journal of Cell Science”

The document concludes that the hair cycle is a complex process involving growth, regression, and rest phases, regulated by various molecular signals.

research Stem Cells in the Hair Follicle Bulge Contribute to Wound Repair but Not to Homeostasis of the Epidermis

1279 citations , November 2005 in “Nature Medicine”

research Keratin 15 Promoter Targets Putative Epithelial Stem Cells in the Hair Follicle Bulge

387 citations , November 2003 in “Journal of Investigative Dermatology”

The K15 promoter effectively targets stem cells in the hair follicle bulge.

research Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34

561 citations , April 2003 in “Journal of Investigative Dermatology”

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