Hair Growth Promoting Effect of Thuja Orientalis Ethanol Extracts on Hair Loss-Induced DBA1J Mice

The study conducted 20 years ago investigated the effects of Thuja orientalis extract (TOS) on hair growth in hair loss-induced DBA1J mice. The extract was found to have no cytotoxic effect on non-stimulated cells, but it significantly reduced the release of pro-inflammatory cytokines such as interleukin-1beta (IL-1β), IL-6, tumour necrosis factor alpha (TNF-α), and nitric oxide (NO) in stimulated RAW264.7 cells. Furthermore, when TOS (500 μg/ml) was applied to DBA 1J/mice, it remarkably promoted hair growth. The data suggested that TOS promotes hair growth in hair loss induced model mice, indicating the potential anti-hair loss activity of Thuja orientalis.