A Guide to Studying Human Dermal Adipocytes In Situ

March 2018 in “ Experimental Dermatology ”

Carina Nicu, J. Pople, Laura Bonsell, Ranjit Bhogal, David M. Ansell, Ralf Paus

dermal white adipose tissue DWAT dermal adipocytes DAs immunohistochemistry immunofluorescence microscopy quantitative immunohistomorphometry lipid content adipocyte identification apoptosis detection adipocyte proliferation in situ hybridization transmission electron microscopy MRI imaging fat cells fat tissue microscopy MRI

Studysummary This practical guide introduces methods to study human dermal white adipose tissue but does not report new clinical results, emphasizing available techniques and staining protocols.

Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer

In 2018, Carina Nicu and colleagues provided a detailed guide for studying human dermal white adipose tissue (DWAT) and dermal adipocytes (DAs) in situ and ex vivo. The guide covered various techniques for analyzing DWAT, such as immunohistochemistry, immunofluorescence microscopy, and quantitative immunohistomorphometry, and detailed the use of specific stains and markers for lipid content and adipocyte identification. It also addressed challenges in DWAT research, including apoptosis detection and adipocyte proliferation, and discussed advanced techniques like in situ hybridization, transmission electron microscopy, and MRI imaging. The review aimed to enhance understanding of DWAT functions and facilitate research in this area, which could lead to targeted medical therapies. The work was supported by the BBSRC and the NIHR Manchester Biomedical Research Centre, with no conflicts of interest reported.

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