research Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles
This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.
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research Cultured follicles offer hope for beating baldness
Growing hair follicles from cultured cells could potentially treat baldness, but more research is needed.
research Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells
This study describes a protocol for generating hair follicle germ-like organoids from human skin cells, which may aid in alopecia drug discovery and understanding hair follicle regeneration.
research Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics
In this study, researchers differentiated human induced pluripotent stem cells into dermal papilla-like cells and observed gene expression dynamics during five stages of dermal differentiation, demonstrating the potential of these cells to interact with epidermal cells in functional assays.
research Epidermal stem cells and skin tissue engineering in hair follicle regeneration
This review discusses current experimental approaches for regenerating human hair follicles using tissue engineering and isolated cells, but reports no successful strategies with adult cells yet found.
research Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid
This study found that while extracellular matrix components like hyaluronic acid promoted larger organoid formation, other matrix components hindered hair follicle germ assembly in an in vitro model.
research Morphogenetic Events in Hair Follicle Heterotypic Spheroids
This study found that hyaluronic acid increased the size of hair follicle germ-like aggregates and the number of proliferative cells but did not maintain specific markers during the process.
research Hair follicle regeneration in vitro
This study developed an in vitro artificial hair germ model using dermal papilla cells and skin keratinocytes to explore early stages of hair follicle regeneration.
research Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration
This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.
research 883 Hair follicle bulb developed as 3D scaffold free microtissue
This study observed that a 3D culture model of hair follicle cells showed an anagen-like phase between days 3 to 6 and transitioned to a catagen-like phase by day 7, highlighting cell differentiation and structural development over time.
research Hair Strengthening Evaluation of Anisotropic Osmolite Solutions (Inositol + Arginine): Cross-Talk between Dermal Papilla Fibroblast and Keratinocytes of the Outer Root Sheath Using a µHair Follicle 3D Model
This study found that anisotropic osmolyte solutions may improve hair follicle turnover and condition in both in vitro and in silico models, as well as in human volunteers with hair disorders.
research Biological activity and hair inducing function of biomimetic dermal papilla spheres prepared by hanging drops of methacrylate gelatin
This study found that biomimetic dermal papilla spheres cultured in a specific microenvironment can partially restore hair-inducing ability in high-passage dermal papilla cells in nude mice, resembling the characteristics of primary cells.
research Generating hair follicle inductive dermal papillae cells from adipose derived mesenchymal stem cells
The study suggests that culturing adipose-derived mesenchymal stem cells into spheroids with Wnt exposure can result in cell clusters resembling hair follicle dermal papilla cells, potentially aiding hair follicle generation.
research Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration
This review discusses recent advances in developmental tissue engineering for regenerating ectodermal appendages like teeth and glands, emphasizing biomaterial selection and cell culture strategies, but reports no new experimental results.
research Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth
This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.
research Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold
The study suggests that a 3D culture system using the RAD16-I peptide scaffold can help restore the original phenotype of hair follicle dermal papilla cells and support their osteogenic and adipogenic differentiation.
research Regenerative medicine and hair loss: how hair follicle culture has advanced our understanding of treatment options for androgenetic alopecia
This review discusses advancements in cell culture techniques for hair growth treatments and provides insights into the potential and challenges of follicular cell implantation, reporting no new clinical results.
research Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay
This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.
research Bioinspired tuning of glycol chitosan for 3D cell culture
This study developed thermo-reversible glycol chitosan hydrogels that effectively form and maintain 3D cell spheroids within one day, offering a simplified method for creating biologically realistic cultures for tissue regeneration and drug screening applications.
research Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture
This study found that forming spheroids using silicone micro-wells improved the viability and neural differentiation potential of human adipose-derived stem cells after thawing.
research RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
This study identified ten key hub genes and pathways crucial for understanding the molecular mechanism of hair growth by comparing dermal papilla cells in 2D and 3D cultures.
research Extracellular vesicles from 3D cultured dermal papilla cells improve wound healing via Krüppel-like factor 4/vascular endothelial growth factor A -driven angiogenesis
This study found that tdDPC-EVs significantly improved wound healing by enhancing angiogenesis through the KLF4/VEGFA axis, offering advantages over traditional DPC-EVs.
research Application and Progress of Cultured Models of Gallbladder Carcinoma
This study examines the development and application of cultured models for gallbladder carcinoma and highlights innovations and challenges in constructing effective in vitro growth models for cancer research.
research Three-dimensional models: from cell culture to Patient-Derived Organoid and its application to future liposarcoma research
This review discusses the potential of 3D cell cultures, particularly Patient-Derived Organoids, in advancing research on liposarcoma by addressing limitations of 2D cultures and animal models but reports no new experimental findings.
research HIF-1α Stimulators Function Equally to Leading Hair Loss Agents in Enhancing Dermal Papilla Growth
This study demonstrated that caffeine, minoxidil, and the HIF-1α-stimulating agent deferiprone significantly enhanced dermal papilla cell proliferation in a 3D culture model, suggesting deferiprone as a potential alternative to minoxidil.
research Laboratory and Experimental Trichology
The document concludes that the trichogram is a useful tool for diagnosing hair loss and suggests semi-organ cultures for practical trichological research.
research Hair Follicle Reconstruction and Stem Cells
This review discusses the regenerative potential of hair follicle stem cell populations and current approaches to reconstructing folliculogenesis for alopecia therapy, but reports no new clinical results.
research The role of 3Rs in the age of One Health: where we are and where we’re going
Innovative methods are reducing animal testing and improving biomedical research.
research The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression
This study isolated a bioactive peptide from Trapa japonica fruit and found that it may protect human dermal papilla cells from DHT-induced stress, suggesting potential for biomedical applications.
research Use of extracellular matrix hydrogel from human placenta to restore hair-inductive potential of dermal papilla cells
This study found that the human placenta extracellular matrix hydrogel restored the hair-inductive capacity of high-passaged dermal papilla cells, enabling them to regenerate new hair follicles when co-grafted with mouse epidermal cells.