Multiomics Global Landscape of Stemness-Related Gene Clusters in Adipose-Derived Mesenchymal Stem Cells

July 2020 in “ Stem Cell Research & Therapy ”

Guanming Lu, Yong-Xian Rong, Zhi-Jie Liang, Dong-Lin Hunag, Yanfei Ma, Zhizhai Luo, Fang-Xiao Wu, Xinheng Liu, Yu Liu, Steven Mo, Zhongquan Qi, Hongmian Li

adipose-derived mesenchymal stem cells stemness ITGAV MAD2L1 PCNA transcription factors lncRNAs KEGG pathways cell proliferation cell differentiation cell migration

TLDR Young donor, early passage stem cells have the highest stemness.

The study investigated the molecular characteristics of adipose-derived mesenchymal stem cells (AD-MSCs) from different passages and donor ages, revealing that stemness was highest in Y1 cells (young donor, passage 3) and lowest in E3 cells (elderly donor, passage 10). Multiomics analysis identified five stemness-related gene clusters linked to cell passages and one linked to age, with key genes ITGAV, MAD2L1, and PCNA showing higher expression in Y1 cells. The study constructed a global regulatory landscape involving 25 transcription factors and 16 lncRNAs regulating 21 KEGG pathways, primarily related to cell proliferation, differentiation, and migration, providing insights for selecting AD-MSCs with enhanced stemness.

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