Changes in the Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cells During Passages
January 2012
in “
Proteome Science
”
TLDR Adipose stem cells show common protein changes as they grow, especially involving S100A6.
The study examined proteomic changes in adipose tissue-derived mesenchymal stem cells (AD-MSC) during cell culture passages using samples from 3 donors. It found significant variability in the proteomic profile both between donors and across different passages, with 41.5% of cytoplasmic and 31.7% of nuclear protein peaks differentially expressed. A key finding was the consistent upregulation of the S100A6 protein in later passages, suggesting its role in cell proliferation and adaptation to culture conditions. These results underscored the need to monitor proteomic changes to ensure the reliability of AD-MSCs for therapeutic applications, as donor variability and passage number significantly influenced protein expression profiles.
