Evaluation of Short-Term Hair Follicle Storage Conditions for Maintenance of RNA Integrity

    May 2024 in “ PLoS ONE ”
    Eilís E. Harkin, John A. Browne, Barbara A. Murphy
    Studysummary This study found that storing horse hair follicles in RNAlater® at cool or room temperature for up to a week did not affect RNA integrity, suggesting it allows for flexible sample transport without immediate freezing.
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    Research cited in this study 11

    1. Hair Follicles as a Critical Model for Monitoring the Circadian Clock International Journal of Molecular Sciences · 2023
    2. Enrichment of Specific Hair Follicle-Associated Cell Populations in Plucked Hairs Offers an Opportunity to Study Gene Expression Underlying Hair Traits International journal of molecular sciences · 2022
    3. Insights Into Male Androgenetic Alopecia Using Comparative Transcriptome Profiling: Hypoxia-Inducible Factor-1 and Wnt/Beta-Catenin Signalling Pathways British Journal of Dermatology · 2022
    4. An Efficient, Non-Invasive Approach for In Vivo Sampling of Hair Follicles: Design and Applications in Monitoring DNA Damage and Aging Aging · 2021
    5. The Effects of Extended Photoperiod and Warmth on Hair Growth in Ponies and Horses at Different Times of Year PloS one · 2020
    6. Human Hair Follicle Transcriptome Profiling: A Minimally Invasive Tool to Assess Molecular Adaptations Upon Low-Volume, High-Intensity Interval Training Physiological Reports · 2017
    7. Transcriptional Profiling in Rat Hair Follicles Following Simulated Blast Insult: A New Diagnostic Tool for Traumatic Brain Injury PLoS ONE · 2014
    8. Technical Note: A Comparison of DNA Collection Methods in Cattle and Yaks Journal of Animal Science · 2014
    9. A Meeting of Two Chronobiological Systems: Circadian Proteins Period1 and BMAL1 Modulate the Human Hair Cycle Clock Journal of Investigative Dermatology · 2013
    10. Investigation of a Non-Invasive Method of Assessing the Equine Circadian Clock Using Hair Follicle Cells Journal of circadian rhythms · 2012
    11. Noninvasive Method for Assessing the Human Circadian Clock Using Hair Follicle Cells Proceedings of the National Academy of Sciences of the United States of America · 2010