Evaluation of Short-Term Hair Follicle Storage Conditions for Maintenance of RNA Integrity

    Eilis Harkin, John Browne, Barbara A. Murphy

    Preprint — not peer reviewed. This was posted to a preprint server or data repository. It has not been through a journal's review process, and its findings may change or not hold up.

    Studysummary In this study, RNA integrity in horse hair follicles stored at various conditions in RNAlater® was maintained, showing that storing them at cool or room temperature for up to a week does not compromise RNA quality or concentration.
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    Research cited in this study 8

    1. Hair Follicles as a Critical Model for Monitoring the Circadian Clock International Journal of Molecular Sciences · 2023
    2. Insights Into Male Androgenetic Alopecia Using Comparative Transcriptome Profiling: Hypoxia-Inducible Factor-1 and Wnt/Beta-Catenin Signalling Pathways British Journal of Dermatology · 2022
    3. An Efficient, Non-Invasive Approach for In Vivo Sampling of Hair Follicles: Design and Applications in Monitoring DNA Damage and Aging Aging · 2021
    4. Human Hair Follicle Transcriptome Profiling: A Minimally Invasive Tool to Assess Molecular Adaptations Upon Low-Volume, High-Intensity Interval Training Physiological Reports · 2017
    5. Transcriptional Profiling in Rat Hair Follicles Following Simulated Blast Insult: A New Diagnostic Tool for Traumatic Brain Injury PLoS ONE · 2014
    6. A Meeting of Two Chronobiological Systems: Circadian Proteins Period1 and BMAL1 Modulate the Human Hair Cycle Clock Journal of Investigative Dermatology · 2013
    7. Investigation of a Non-Invasive Method of Assessing the Equine Circadian Clock Using Hair Follicle Cells Journal of circadian rhythms · 2012
    8. Noninvasive Method for Assessing the Human Circadian Clock Using Hair Follicle Cells Proceedings of the National Academy of Sciences of the United States of America · 2010