Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

February 2013 in “ Journal of Visualized Experiments ”

Wei‐Meng Woo, Scott X. Atwood, Hanson H. Zhen, Anthony E. Oro

TLDR The method quickly analyzes hair growth genes and shows that blocking Smo in skin cells stops hair growth.

The document from 2013 outlines a method for rapid genetic analysis of epithelial-mesenchymal signaling in hair regeneration using a modified chamber hair assay. This method involves isolating primary mouse keratinocytes and dermal cells, manipulating them with lentivirus containing shRNA or cDNA for gene knockdown or overexpression, and then grafting these cells onto nude mice to form hair follicles. The study demonstrated that knocking down Smoothened (Smo) in dermal cells resulted in inhibited hair growth, highlighting the importance of the Shh signaling pathway in hair follicle development. The method allows for the observation of hair follicle morphogenesis within three weeks, offering a faster alternative to traditional genetic models. The success of the assay depends on achieving high viral infection efficiency and the desired level of gene knockdown or overexpression prior to grafting.

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6 / 6 results

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7 / 7 results

research Epithelial-Mesenchymal Interaction in Hair Regeneration and Skin Wound Healing

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research Hair Follicle Regeneration In Vitro

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Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Cited in this study

research Shh Maintains Dermal Papilla Identity and Hair Morphogenesis via a Noggin–Shh Regulatory Loop

research Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences Between Sox2-Positive and Sox2-Negative Cells In Vitro and In Vivo

research Comparisons of Mouse Models for Hair Follicle Reconstitution

research MIM and Cortactin Antagonism Regulates Ciliogenesis and Hedgehog Signaling

research Self-Assembly of Dermal Papilla Cells into Inductive Spheroidal Microtissues on Poly(Ethylene-Co-Vinyl Alcohol) Membranes for Hair Follicle Regeneration

research Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells

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