Proteomic Tools for the Investigation of Human Hair Structural Proteins and Evidence of Weakness Sites on Hair Keratin Coil Segments

October 2011 in “ Analytical biochemistry ”

Nicolas R. Barthélemy, Audrey Bednarczyk, Christine Schaeffer-Reiss, Dominique Jullien, Alain Van Dorsselaer, Nükhet Cavusoglu

proteomic mass spectrometry multidimensional protein identification technology MudPit two-dimensional gel electrophoresis 2-DE gel MS human hair cortex proteins keratins keratin-associated proteins KAPs posttranslational modifications cysteine trioxidation lysine methylation histidine methylation α-helical segments hair keratins proteomic MS hair proteins cysteine oxidation alpha-helical segments

TLDR Hair proteins have weak spots in their α-helical segments.

The study utilized proteomic mass spectrometry (MS)-based strategies, specifically multidimensional protein identification technology (MudPit) and two-dimensional gel electrophoresis coupled with MS (2-DE gel MS), to analyze human hair cortex proteins. These methods identified all keratins and major keratin-associated proteins (KAPs), along with posttranslational modifications such as cysteine trioxidation, lysine, and histidine methylation. The 2-DE gel MS revealed unexpected heterogeneity in keratin structures and suggested a preferential breaking zone in α-helical segments of hair keratins, providing insights into the structural weaknesses of hair proteins.

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Research cited in this study

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Proteomic Tools for the Investigation of Human Hair Structural Proteins and Evidence of Weakness Sites on Hair Keratin Coil Segments

Research cited in this study

research Characterization of Low Abundance Wool Proteins Through Novel Differential Extraction Techniques

research Transglutaminase-3 Enzyme: A Putative Actor in Human Hair Shaft Scaffolding?

research Characterization of Human KAP24.1, A Cuticular Hair Keratin-Associated Protein with Unusual Amino Acid Composition and Repeat Structure

research Hair: The Most Sophisticated Biological Composite Material

research Sequence Analyses of Type I and Type II Chains in Human Hair and Epithelial Keratin Intermediate Filaments: Promiscuous Obligate Heterodimers, Type II Template for Molecule Formation and a Rationale for Heterodimer Formation

research Human Hair Keratin-Associated Proteins: Sequence Regularities and Structural Implications

research Proteome Analysis of Human Hair Shaft

research Human Hair Keratin-Associated Proteins

research Transglutaminase 5 Expression in Human Hair Follicle

research Keratins of the Human Hair Follicle

research Hair Keratin Associated Proteins: Characterization of a Second High Sulfur KAP Gene Domain on Human Chromosome 21 in Fond Memory of Dr. Peter Steinert

research Human Hair: A Unique Physicochemical Composite

research Hard and Soft Principles Defining the Structure, Function, and Regulation of Keratin Intermediate Filaments

research The Catalog of Human Hair Keratins

research Hair Structure Anatomy And Comparative Anatomy Dedicated To The Memory Of The Late Dr. R. E. Rivett Whose Untimely Death Prevented His Role In Co-Authorship

research The Catalog of Human Hair Keratins

research Hair: Its Structure and Response to Cosmetic Preparations

research Structure and Biochemistry of Mammalian Hard Keratin

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