Correlation of Proteolytic Activities of Organ Cultured Intact Mouse Skin With Defined Hair Cycle Stages

June 1994 in “ Journal of Dermatological Science ”

Ralf Paus, Niels Krejci‐Papa, Lingna Li, Beate M. Czarnetzki, Robert M. Hoffman

proteolytic activities hair cycle stages collagenase anagen telogen zymography interstitial collagenase gelatinases TPA TNF-α C57 BL-6 mouse model tumor necrosis factor alpha 12-O-tetradecanoylphorbol-13-acetate

TLDR Proteolytic activity in mouse skin changes with hair cycle stages, peaking in early anagen.

The study investigated the proteolytic activities in organ-cultured intact mouse skin across different hair cycle stages. It found that proteolytic activities varied with the hair cycle, with early anagen skin showing significant collagenase activity, while telogen and mid-anagen skin showed minimal activity. The study used zymography to identify the secretion of activated interstitial collagenase and gelatinases, with the highest collagenase activity in anagen IV skin. The addition of TPA or TNF-α enhanced collagenase type I activity. The C57 BL-6 mouse model was highlighted as a valuable tool for studying hair cycle-associated proteolysis.

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