Optimization of Polyphenol Extraction from Eclipta Prostrata L. by Response Surface Methodology

    Thai Quang Nguyen, Vy N. T. Duong, Thi Phuong Hang Tran, Tao Su, Nguyễn Thị Ngọc Lan
    TLDR The best way to extract antioxidants from Eclipta prostrata is using a 43:1 solvent ratio at 64°C for 97 minutes.
    This study optimized the extraction of polyphenols from Eclipta prostrata L., a medicinal herb known for its antioxidant, anti-inflammatory, and antibacterial properties. Using response surface methodology, the optimal conditions were determined to be a solvent:material ratio of 43:1 mL/g, at 64°C for 97 minutes, resulting in a polyphenol content of 55.92 mg GAE/g dw. The extract demonstrated significant antioxidant activity with an IC50 value of 78 µg/mL, as measured by the DPPH free radical scavenging method.
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