Optimization of Polyphenol Extraction from Eclipta Prostrata L. by Response Surface Methodology

This study optimized the extraction of polyphenols from Eclipta prostrata L., a medicinal herb known for its antioxidant, anti-inflammatory, and antibacterial properties. Using response surface methodology, the optimal conditions were determined to be a solvent:material ratio of 43:1 mL/g, at 64°C for 97 minutes, resulting in a polyphenol content of 55.92 mg GAE/g dw. The extract demonstrated significant antioxidant activity with an IC50 value of 78 µg/mL, as measured by the DPPH free radical scavenging method.