Polyamine Oxidase Expression Is Downregulated by 17β-Estradiol via Estrogen Receptor 2 in Human MCF-7 Breast Cancer Cells
July 2022
in “
International Journal of Molecular Sciences
”
17β-estradiol estrogen receptor 2 polyamine oxidase PAOX MCF-7 breast cancer cells estrogen receptor-positive mRNA protein levels ESR2 selective ESR antagonists knockdown luciferase reporter assay promoter activity activator protein 1 AP-1 chromatin immunoprecipitation transcription estrogen deficiency estrogen ER2 breast cancer cells protein estrogen receptor antagonists gene knockdown reporter assay gene promoter gene transcription hormone deficiency
Studysummary This study found that in estrogen receptor-positive breast cancer cells, 17β-estradiol repressed polyamine oxidase transcription by interacting with AP-1 sites on its promoter. Our plain-language summary of this paper — not a Tressless recommendation.
The study found that 17β-estradiol (E2) significantly downregulated polyamine oxidase (PAOX) expression in MCF-7 breast cancer cells through estrogen receptor 2 (ESR2). E2 reduced PAOX mRNA and protein levels, with a 4.3-fold decrease in mRNA, by binding to AP-1 sites on the PAOX promoter. This repression was independent of estrogen response elements and involved the E2–ESR2 complex interacting with AP-1 components. The study suggested that estrogen deficiency could lead to increased PAOX expression and decreased polyamine levels, potentially affecting conditions like hair loss. The findings were based on experiments with a sample size of 3, using methods such as PCR, Western blotting, and luciferase assays.
