Polyamine Oxidase Expression Is Downregulated by 17β-Estradiol via Estrogen Receptor 2 in Human MCF-7 Breast Cancer Cells
July 2022
in “
International Journal of Molecular Sciences
”
17β-estradiol estrogen receptor 2 polyamine oxidase PAOX MCF-7 breast cancer cells estrogen receptor-positive mRNA protein levels ESR2 selective ESR antagonists knockdown luciferase reporter assay promoter activity activator protein 1 AP-1 chromatin immunoprecipitation transcription estrogen deficiency estrogen ER2 breast cancer cells protein estrogen receptor antagonists gene knockdown reporter assay gene promoter gene transcription hormone deficiency
TLDR 17β-estradiol lowers polyamine oxidase levels in breast cancer cells through estrogen receptor 2.
The study found that 17β-estradiol (E2) significantly downregulated polyamine oxidase (PAOX) expression in MCF-7 breast cancer cells through estrogen receptor 2 (ESR2). E2 reduced PAOX mRNA and protein levels, with a 4.3-fold decrease in mRNA, by binding to AP-1 sites on the PAOX promoter. This repression was independent of estrogen response elements and involved the E2–ESR2 complex interacting with AP-1 components. The study suggested that estrogen deficiency could lead to increased PAOX expression and decreased polyamine levels, potentially affecting conditions like hair loss. The findings were based on experiments with a sample size of 3, using methods such as PCR, Western blotting, and luciferase assays.
