Methods and Models for the Investigation of the Uptake of Nanoparticles into Hair Follicles

January 2014 in “ SciDok (Saarland University and State Library) ”

Raber, Anne Susanne

nanoparticles hair follicles transfollicular vaccination poly(lactide-co-glycolide) PLGA chitosan-PLGA ovalbumin CD4+ T-cells CD8+ T-cells phospholipid coatings NPs OVA

Studysummary This study demonstrated that in vitro pig ear models effectively predict human follicular uptake of nanoparticles for potential transfollicular vaccination, but massage did not enhance nanoparticle penetration. Our plain-language summary of this paper — not a Tressless recommendation.

The document discussed the development of in vitro skin models and methods to investigate the uptake of nanoparticles (NPs) into hair follicles, with a focus on their potential for transfollicular vaccination. A method was created to quantify NP deposition in hair follicles, showing a strong correlation between in vitro pig ear models and in vivo human forearm results. Surface modifications, such as phospholipid coatings on poly(lactide-co-glycolide) (PLGA) NPs, were found to affect penetration levels, although massage increased penetration depth but not the amount of NPs in follicles. A proof of concept study demonstrated that PLGA and chitosan-PLGA NPs loaded with ovalbumin stimulated CD4+ and CD8+ T-cells more effectively than ovalbumin in solution, and transfollicular vaccination was possible in mice without prior skin treatment.

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