Mitogen-Regulated Protein/Proliferin mRNA Induction Following Single Applications of Tumor Promoters to Murine Skin

    May 2005 in “ Molecular Carcinogenesis
    Craig L.J. Parfett
    TLDR mrp/plf-mRNA can indicate tumor-promoting effects in skin.
    The study investigated the response of mitogen-regulated protein/proliferin (mrp/plf) mRNA to tumor promoters in murine skin. Researchers applied tumor promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and benzoyl peroxide (BPO), to the skin of SENCAR mice and analyzed RNA samples from treated areas. They found that mrp/plf-mRNA was not detected in Northern blot hybridizations, but significant increases in mRNAs for genes like ornithine decarboxylase and junB were observed in response to TPA. Reverse transcriptase-polymerase chain reactions revealed fully-processed MRP/plf-mRNA in most TPA-treated and some BPO-treated animals, with predominant expression of plf1 and mrp3. The study concluded that mrp/plf-mRNA was differentially expressed in response to tumor promoters and could serve as a biomarker for tumor-promoting effects in chemical carcinogenesis.
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