Induction of Dark Keratinocytes by 12-O-Tetradecanoyl-Phorbol-13-Acetate and Mezerein as an Indicator of Tumor-Promoting Efficiency

January 1980 in “ Carcinogenesis ”

Andres J. Klein‐Szanto, Suzanne K. Major, Thomas J. Slaga

12-O-tetradecanoyl-phorbol-13-acetate mezerein dark keratinocytes tumor-promoting effects cell proliferation basal layer of the skin TPA MZ

TLDR TPA is about 50 times more effective at promoting tumors than MZ.

In a 1980 study by Klein-Szanto, Major, and Slaga, the tumor-promoting effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and mezerein (MZ) were compared by examining their ability to induce dark keratinocytes in mouse skin. TPA was found to induce significantly more dark cells than MZ, with a maximum of 21% increase in the basal layer of the skin 24 hours after a single application of 4 µg of TPA. Both TPA and MZ increased the labeling indices, indicating enhanced cell proliferation. The study, which used 141 mice and observed effects up to 144 hours post-treatment, concluded that the production of dark cells is an important early event in tumor promotion and that TPA is approximately 50 times more effective as a tumor promoter than MZ.

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