Induction of Dark Keratinocytes by 12-O-Tetradecanoyl-Phorbol-13-Acetate and Mezerein as an Indicator of Tumor-Promoting Efficiency

January 1980 in “ Carcinogenesis ”

Andres J. Klein‐Szanto, Suzanne K. Major, Thomas J. Slaga

12-O-tetradecanoyl-phorbol-13-acetate mezerein dark keratinocytes tumor-promoting effects cell proliferation basal layer of the skin TPA MZ

TLDR TPA is about 50 times more effective at promoting tumors than MZ.

In a 1980 study by Klein-Szanto, Major, and Slaga, the tumor-promoting effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and mezerein (MZ) were compared by examining their ability to induce dark keratinocytes in mouse skin. TPA was found to induce significantly more dark cells than MZ, with a maximum of 21% increase in the basal layer of the skin 24 hours after a single application of 4 µg of TPA. Both TPA and MZ increased the labeling indices, indicating enhanced cell proliferation. The study, which used 141 mice and observed effects up to 144 hours post-treatment, concluded that the production of dark cells is an important early event in tumor promotion and that TPA is approximately 50 times more effective as a tumor promoter than MZ.

View this study on academic.oup.com →

Discuss this study in the Community →

Related Community Posts Join

6 / 1000+ results

community I am a dermatologist with a clinical interest in alopecia. AMA

in Will treatment work for me? 2 years ago

In this conversation, 4990 discussed various treatments for hair loss, including oral minoxidil, PRP, transplan, Jak inhibitors, Dutasteride, Finasteride, Olumiant, Ketoconazole, RU58841, microneedling, baricitinib, and CCCA. They recommended scalp biopsies in unclear cases of DUPA, twice weekly to twice daily shampooing for topical minoxidil users, and two sessions spaced one month apart with follow up at month three to determine the effectiveness of PRP treatment.

community 1.5 years on fin and min (24 yo). Start early guys, it’s worth it.

in Progress Pictures 1167 upvotes 1 year ago

The conclusion of the conversation is that the user "CheckHopeful" has seen significant improvement in their hair loss after using finasteride and minoxidil for 1.5 years. Some users discuss side effects and suggest adjusting the dosage or trying topical finasteride or RU58841.

community Only 10 more days until cassiopea releases their 12 month results for CB-03-01 our best hope of an alternative to finasteride

in Finasteride 155 upvotes 6 years ago

The conversation discusses anticipation for CB-03-01, a potential new hair loss treatment that may be more effective and have a cleaner safety profile than finasteride. Users are hopeful but cautious, discussing current treatments like minoxidil, nizoral, and dermarolling, and the possibility of combining them with CB-03-01 for better results.

community Just popped my first fin, excited as hell!

in Finasteride/Dutasteride 154 upvotes 9 months ago

A user shared their excitement about starting finasteride for hair loss after trying various ineffective methods. Other users discussed their experiences with finasteride, including shedding phases and side effects, with one mentioning using topical finasteride and minoxidil.

community 12 months Minoxidil twice a day, 5 months Dermarolling, 11 months Finasteride 1mg a day, started at 25 y/o, sadly not that good photos from before starting, you can open photos on new tab and zoom. First 6 photos are from before, remaining from after, I have photos from between if anyone wants.

in Progress Pictures 34 upvotes 9 months ago

The conversation is about a person's 12-month hair loss treatment with Minoxidil twice daily, Dermarolling for 5 months, and Finasteride 1mg daily for 11 months, starting at age 25, with progress photos shared. The before and after photos show the treatment results, and additional photos are available upon request.

community Male - 25 y.o - gulfs slowly fading like my father’s - topical minoxidil, soon starting fin + keratine supplement

in Journals / Progress Pictures 231 upvotes 7 months ago

A 25-year-old male is using topical minoxidil and plans to start finasteride and a keratin supplement to combat hair loss. He will document his progress with photos and track any side effects.

Similar Research

5 / 1000+ results

Tumor Promotion in Skin: Are Active Oxygen Species Involved?

research Induction of Ornithine Decarboxylase in Specific Subpopulations of Murine Epidermal Cells Following Multiple Exposures to 12-O-Tetradecanoylphorbol-13-Acetate, Mezerein, and Ethyl Phenylpropriolate

26 citations, January 1992 in “Carcinogenesis”

TPA strongly increases ODC activity in certain skin cells, potentially aiding tumor growth.