The Mammalian Hairless Protein as a DNA Binding Phosphoprotein

June 2016 in “ Journal of cellular biochemistry ”

Lemlem Brook, G. Kerr Whitfield, David Hsieh, Ryan D. Bither, Jui Cheng Hsieh

Studysummary This study found that the mammalian Hr protein can interact with the p53 pathway by binding to a specific p53 response element, influencing the regulation of genes involved in cell cycle control.

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The study investigated the mammalian hairless (Hr) protein, revealing its specific binding to a p53 response element (p53RE) and its interaction with the p53 protein, which influences the regulation of cell cycle control genes GADD45A and p21. Chromatin immunoprecipitation (ChIP) assays confirmed HR binding to the p53RE of the GADD45A gene in HEK293 and U87 cells. Co-immunoprecipitation assays showed direct interaction between HR and p53. Additionally, Hr was found to be a substrate for PKC but not PKA, with human HR phosphorylated at Ser-416 in U87 cells. These findings suggested that Hr is a phosphoprotein involved in the p53 pathway, impacting cell proliferation and differentiation in skin and brain tissues.

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research Hairless Plays a Role in Formation of Inner Root Sheath via Regulation of Dlx3 Gene

17 citations , March 2012 in “Journal of biological chemistry/The Journal of biological chemistry”

In this study, researchers found that overexpression of the hairless protein in Hr mutant mice disrupts inner root sheath formation by downregulating Dlx3 and associated keratins in hair follicle development.

research Analysis of Hairless Corepressor Mutants to Characterize Molecular Cooperation with the Vitamin D Receptor in Promoting the Mammalian Hair Cycle

22 citations , April 2010 in “Journal of Cellular Biochemistry”

This study concluded that the hairless protein interacts with the vitamin D receptor to repress transcription crucial for hair cycling, employing multiple protein interfaces and modulating chromatin structure.

research Hairless Is a Nuclear Receptor Corepressor Essential for Skin Function

30 citations , January 2009 in “Nuclear Receptor Signaling”

This study identified the Hairless (Hr) gene-encoded protein as a corepressor that plays a crucial role in maintaining skin and hair by regulating epithelial stem cell differentiation and gene expression via chromatin remodeling, which may impact both development and disease.

research Physical And Functional Interaction Between The Vitamin D Receptor And Hairless Corepressor, Two Proteins Required For Hair Cycling

215 citations , September 2003 in “Journal of Biological Chemistry”

This study found that the hairless gene product (Hr) suppresses VDR-mediated gene activation by directly interacting with the vitamin D receptor, potentially affecting hair follicle function.

research The Hairless Gene Mutated in Congenital Hair Loss Disorders Encodes a Novel Nuclear Receptor Corepressor

178 citations , October 2001 in “Genes & Development”

This study found that the mammalian hairless gene encodes a corepressor protein that interacts with thyroid hormone receptors, providing insights into hair loss syndromes in humans and mice.

research A Novel Missense Mutation (C622G) in the Zinc-Finger Domain of the Human Hairless Gene Associated with Congenital Atrichia with Papular Lesions

47 citations , April 2000 in “Experimental Dermatology”

This study identified a novel missense mutation in the hairless gene in a Polish family, which may contribute to congenital atrichia with papular lesions by affecting the gene's function.

research A Missense Mutation in the Zinc-Finger Domain of the Human Hairless Gene Underlies Congenital Atrichia in a Family of Irish Travellers

83 citations , October 1998 in “The American Journal of Human Genetics”

A specific gene mutation causes complete hair loss in an Irish Traveller family.