Isotope Dilution-Based Targeted and Nontargeted Carbonyl Neurosteroid/Steroid Profiling

March 2018 in “ Analytical chemistry ”

Sheila Sharp, Stephen J. Mitchell, Monique Vallée, Elena Kuzmanova, Michelle A. Cooper, Delia Belelli, Jeremy J. Lambert, Jeffrey Huang

finasteride pregnenolone progesterone 3α,5α-tetrahydroprogesterone neurosteroids steroids isotope dilution hydroxylamine derivatization mass spectrometry MS/MS analysis Propecia

Studysummary This study found that a new analytical approach revealed an increased brain pregnenolone level following finasteride treatment in a mouse model, suggesting potential for improved understanding of neurosteroid roles in brain excitability.

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