Combining Laser Capture Microdissection and RNAseq Analysis to Generate a Human Anagen Hair Follicle Transcriptome Atlas
December 2022
in “
The journal of investigative dermatology/Journal of investigative dermatology
”
anagen hair follicles laser capture microdissection RNA sequencing bulge outer root sheath suprabulbar outer root sheath inner root sheath precortical hair matrix germinative hair matrix dermal papilla lower dermal cup connective tissue sheath principal component analysis signature genes gene expression profiles targeted therapies hair follicle disorders anagen HFs LCM RNAseq ORS IRS HM DP DC CTS PCA
TLDR Researchers made a detailed map of gene activity for different parts of human hair follicles to help create targeted hair disorder treatments.
Researchers aimed to create a detailed transcriptome atlas of human anagen hair follicles (HFs) by performing laser capture microdissection (LCM) on 8 specific HF compartments and analyzing them using RNA sequencing (RNAseq). The compartments included the bulge outer root sheath (ORS), suprabulbar ORS, inner root sheath (IRS), precortical hair matrix (HM), germinative HM, dermal papilla (DP), lower dermal cup (DC), and connective tissue sheath (CTS). Principal component analysis (PCA) confirmed the distinctness of these compartments, and known signature genes were correctly matched to their respective compartments. This data was used to create a publicly accessible database for identifying compartment-specific gene expression profiles, which can aid in developing targeted therapies for human HF disorders.
