Hair Follicle Associated Pluripotent Stem Cells Stimulate Hair Growth in Transplanted Mice

December 2022 in “ Scientific Reports ”

Koya Obara, Jose Reynoso, Yuko Hamada, Yusuke Aoki, Yutaro Kubota, Noriyuki Masaki, Yasuyuki Amoh, Robert M. Hoffman

Hair follicle associated pluripotent stem cells HAP stem cells hair growth transplantation athymic nu/nu nude mice FOXN1 gene hair follicles cell therapy hair-growth area hair length hair-density score stem cells nude mice

Studysummary This study found that transplanted hair follicle-associated pluripotent stem cells in mice differentiated into keratinocytes and stimulated hair growth by producing mature hair shafts.

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The study demonstrated that Hair follicle associated pluripotent (HAP) stem cells, taken from the upper parts of whisker follicles in mice, can stimulate hair growth when transplanted into the skin of athymic nu/nu nude mice, which are unable to produce mature hair shafts due to a mutation in the FOXN1 gene. The transplanted HAP stem cells migrated to the resident hair follicles of the recipient mice, promoting hair growth. The transplanted mice showed significant increases in hair-growth area, hair length, and hair-density score compared to non-transplanted control mice. The study suggests that HAP stem cells could potentially be used in cell therapy for hair growth stimulation, but also noted that repeated transplantation may be necessary to maintain hair growth. The number of mice used in the experiment was not mentioned.

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8 citations , May 2021 in “Bioengineering & translational medicine”

This review examines the challenges of hair follicle regeneration and outlines strategies for bioengineering human hair follicle models, without presenting new experimental results.

research Rescuing Key Native Traits in Cultured Dermal Papilla Cells for Human Hair Regeneration

30 citations , November 2020 in “Journal of Advanced Research”

This study found that conditioned medium from keratinocytes improved the hair-inductive properties of cultured dermal papilla cells, suggesting potential for enhancing hair follicle regeneration.

Autologous Cell-Based Therapy for Male and Female Pattern Hair Loss Using Dermal Sheath Cup Cells: A Randomized Placebo-Controlled Double-Blinded Dose-Finding Clinical Study

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22 citations , March 2020 in “Journal of The American Academy of Dermatology”

This study found that injecting autologous dermal sheath cup cells significantly increased hair density and diameter in male and female pattern hair loss compared to a placebo for up to 9 months.

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40 citations , June 2019 in “Biochemical and Biophysical Research Communications”

This study demonstrated that combining the Wnt/β-catenin activator CHIR99021 with 3D spheroid culture effectively enriched hair follicle formation in reconstituted human skin.

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77 citations , April 2016 in “Science Advances”

Researchers created a fully functional, bioengineered skin system with hair from stem cells that successfully integrated when transplanted into mice.

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62 citations , February 2016 in “ACS Applied Materials & Interfaces”

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

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64 citations , May 2015 in “Cell Cycle”

This study found that hair follicle stem cells from mice can differentiate into beating cardiac muscle cells, suggesting potential applications for heart regeneration in regenerative medicine.

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184 citations , November 2014 in “Developmental Cell”

This study identifies a bipotent stem cell in adult hair follicles that self-renews and contributes to dermal sheath and papilla cell populations, potentially aiding hair regrowth after injury, disease, or aging.

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256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

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78 citations , October 2012 in “Biomaterials”

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

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47 citations , August 2012 in “Cell Cycle”

This study found that multipotent, nestin-expressing stem cells from the upper part of hair follicles can proliferate significantly and differentiate into various cell types, showing potential for nerve and spinal cord repair.

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66 citations , May 2012 in “Scientific Reports”

This study demonstrated that bioengineered hair follicles reconstituted from embryonic skin cells and transplanted into hosts can restore physiological hair functions, suggesting potential applications for treating alopecia.

research Review of Hair Follicle Dermal Cells

321 citations , December 2009 in “Journal of Dermatological Science”

This review discusses the role of dermal cells in hair follicle regeneration and morphogenesis and highlights their potential therapeutic implications, but it reports no new empirical findings.

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419 citations , March 2005 in “Proceedings of the National Academy of Sciences”

This study demonstrated that ND-GFP stem cells from the hair-follicle bulge area can differentiate into various cell types, including neurons, suggesting their potential as a source for therapeutic applications.

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This study found that nestin-driven GFP labels blood vessels originating from hair follicles in mice, which could be used to study early events in skin angiogenesis and for antiangiogenesis drug screening.

Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

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208 citations , December 2003 in “Journal of Investigative Dermatology”

This study found that dermal papilla and peribulbar dermal sheath cup cells in mice can both induce hair follicle formation, suggesting they may have similar functional roles in hair growth.

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352 citations , August 2003 in “Proceedings of the National Academy of Sciences”

In this study, nestin-expressing cells in the hair follicle bulge, marked by GFP in transgenic mice, were identified as progenitors of the hair follicle outer-root sheath.

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